Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

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dc.authorid0000-0003-3676-5147
dc.authorid0000-0001-5170-5594
dc.authorid0000-0001-5170-5594
dc.authorid0000-0001-7256-0750
dc.authorscopusid57214892268
dc.authorscopusid6603585540
dc.authorscopusid56018882300
dc.authorscopusid8283704400
dc.authorscopusid16836077200
dc.authorwosidÖztekin, Nevin/F-7653-2018
dc.authorwosidCansever, Mehmet Serif/AAU-8758-2020
dc.authorwosidKIYKIM, Ertugrul/ABA-9964-2020
dc.authorwosidKIYKIM, Ertugrul/D-1332-2019
dc.authorwosidZeybek, Ayse Cigdem Aktuglu/C-9812-2019
dc.contributor.authorÖzçelik, Sirun
dc.contributor.authorÖztekin, Nevin
dc.contributor.authorKıykım, Ertuğrul
dc.contributor.authorCansever, Mehmet Şerif
dc.contributor.authorAktuğlu Zeybek, Ayse Çiğdem
dc.date.accessioned2022-05-11T14:47:20Z
dc.date.available2022-05-11T14:47:20Z
dc.date.issued2020
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractEthylmalonic acid is a metabolic organic acid, and its accumulation in urine is diagnostic of ethylmalonic aciduria. In this study, a simple and fast method employing capillary electrophoresis equipped with capacitively coupled contactless conductivity detection was developed for the detection of ethylmalonic acid in urine samples. The optimized electrophoretic separation was performed in 50 mmol/L 2-(N-morpholino)ethanesulfonic acid solution, buffered at a pH of 6.5, and contained 0.13 mmol/L cetyltrimethylammonium bromide as an electroosmotic modifier. Electrophoresis was run at 28 kV in reversed polarity. The linear range of ethylmalonic acid concentration was between 1 and 100 mg/L with a regression coefficient of 0.9998. This method had good intra- and interday precision with <5% relative standard deviations. The detection limit (signal-to-noise ratio = 3) and the quantification limit (signal-to-noise ratio = 10) values were 0.139 and 0.466 mg/L, respectively. Using our optimized conditions, the method was successfully employed for the detection of ethylmalonic acid in urine sample of ethylmalonic aciduria patient.
dc.description.sponsorshipResearch Foundation of Istanbul Technical UniversityIstanbul University [TDK-2018-41370]
dc.description.sponsorshipResearch Foundation of Istanbul Technical University, Grant/Award Number: TDK-2018-41370
dc.identifier.doi10.1002/jssc.201901044
dc.identifier.endpage1371
dc.identifier.issn1615-9306
dc.identifier.issn1615-9314
dc.identifier.issue7en_US
dc.identifier.pmid31958360
dc.identifier.scopus2-s2.0-85079465140
dc.identifier.scopusqualityQ2
dc.identifier.startpage1365
dc.identifier.urihttps://doi.org/10.1002/jssc.201901044
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10476
dc.identifier.volume43
dc.identifier.wosWOS:000512077900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansever, Mehmet Şerif
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofJournal of Separation Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectethylmalonic encephalopathy
dc.subjectinborn error
dc.subjectmetabolic disorders
dc.subjecturine
dc.subjectOrganic-Acids
dc.subjectMass-Spectrometry
dc.subjectBiological-Fluids
dc.subjectEncephalopathy
dc.subjectMetabolism
dc.subjectChromatography
dc.subjectFormate
dc.subjectBlood
dc.subjectSerum
dc.titleCapillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria
dc.typeArticle

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