Humic Acid Has Protective Effect on Gastric Ulcer by Alleviating Inflammation in Rats

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dc.authorscopusid36115476100
dc.authorscopusid57194872315
dc.authorscopusid55838809000
dc.authorscopusid55897728300
dc.authorscopusid7003873872
dc.contributor.authorŞehitoğlu, Müşerref Hilal
dc.contributor.authorÖztopuz, Ö.
dc.contributor.authorKaraboğa, İhsan
dc.contributor.authorOvalı, M. A.
dc.contributor.authorUzun, Metin
dc.date.accessioned2022-05-11T14:48:38Z
dc.date.available2022-05-11T14:48:38Z
dc.date.issued2022
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Acil Yardım ve Afet Yönetimi Bölümü
dc.description.abstractThe new agents are needed in treatment of gastric ulcer that have less side effects, adequate efficacy, and no drug interactions. In this study, we aimed to investigate the potential protective effects of humic acid on experimental gastric ulcer. Wistar Albino male rats (n = 48) were randomly divided into 8 groups as follow; Control (without any applications), Humic acid (50 mg/kg), ethanol group (1 mL/rat), and indomethacin group (25 mg/kg). In the treatment groups, both gastric ulcer model and humic acid 50 mg/kg were applied. In addition, famotidine the antiulcer drug was used as positive control. All medications were administered by oral gavage. Levels of ADAM10 and ADAMTS12 in gastric mucosa were determined by ELISA method. Hematoxylin-Eosin (H&E) staining, iNOS, and PCNA immunohistochemical staining were performed for histopathological investigations. Apoptosis was demonstrated by using the TUNEL method. In addition, the levels of inflammatory cytokines (TNF-?, IL-6, IL-10) and caspase-3 gene were determined by qRT-PCR. ADAM10 and ADAMTS12 levels significantly increased in the treatment groups compared to the ulcer groups (p < 0.05). The experimental groups showed mucosal erosion, bleeding, leukocyte infiltration and edema. Treatment with humic acid and famotidine was found to suppress iNOS activity, thereby decreasing proinflammatory activity and preventing damage to the gastric mucosa, while reducing the number of apoptotic cells. IL-6, IL-10, TNF-? and caspase-3 levels were significantly decreased in the treatment groups compared to damaged gastric mucosa. As a result, humic acid may be defined as a potential protective agent with its anti-inflammatory effect in gastric ulcer. © 2022, Allerton Press, Inc.
dc.description.sponsorshipTSA-2018-2592
dc.description.sponsorshipThis study was supported by the Scientific Research Commission of Canakkale Onsekiz Mart University with the project named “Investigation of the Effects of Humic Acid in Different Acute Gastric Ulcer Models in Rats” coded TSA-2018-2592.
dc.identifier.doi10.3103/S0095452722010091
dc.identifier.endpage97
dc.identifier.issn0095-4527
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85124947531
dc.identifier.scopusqualityQ4
dc.identifier.startpage84
dc.identifier.urihttps://doi.org/10.3103/S0095452722010091
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10666
dc.identifier.volume56
dc.indekslendigikaynakScopus
dc.institutionauthorKaraboğa, İhsan
dc.language.isoen
dc.publisherPleiades journals
dc.relation.ispartofCytology and Genetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectADAM10
dc.subjectADAMTS12
dc.subjectfamotidine
dc.subjecthumic acid
dc.subjectinflammation
dc.subjectKeywords: gastric mucosa injury
dc.titleHumic Acid Has Protective Effect on Gastric Ulcer by Alleviating Inflammation in Rats
dc.typeArticle

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