ARID3A-mediated modulation of TP73 and TP73-AS1 in osteosarcoma cells

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dc.authorscopusid55815636400
dc.authorscopusid56497061100
dc.authorscopusid56190917500
dc.authorscopusid36772919600
dc.authorscopusid35482179000
dc.contributor.authorSaadat, Khandakar A.S.M.
dc.contributor.authorBozgeyik, Esra
dc.contributor.authorArman, Kaifee
dc.contributor.authorBozgeyik, İbrahim
dc.contributor.authorİkeda, M.A.
dc.date.accessioned2022-05-11T14:05:11Z
dc.date.available2022-05-11T14:05:11Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractOsteosarcoma is the most common primary malignancy arising from bone. Increasing mass of indications suggest that long non-coding RNAs (lncRNAs) play crucial roles in the development of progressions of human cancers including osteosarcoma. Although several lncRNAs have shown to be involved in the molecular pathogenesis of osteosarcoma, identification of novel lncRNAs involved in the osteosarcoma pathobiology remained muchly elusive. Besides, ARID3A is a member of ARID family of DNA binding proteins. ARID3A was also implicated in osteosarcoma pathogenesis. Accordingly, in the present study, we mechanistically investigated the effect of ARID3A on the expression of TP73 and TP73-AS1 in osteosarcoma cells and to determine the relationship between them. For the overexpression of ARID3A in U2-OS cells, pER_xpress_ARID3A expression vector and for the silencing of ARID3A, ARID3A-spesific siRNA was used. Expression levels of ARID3A, TP73 and TP73-AS1 genes were determined by qPCR method. As a result, expression levels of TP73-AS1 were well-correlated with the ARID3A expression levels whereas TP73 expression levels were not well-correlated with ARID3A. In conclusion, our results indicate that ARID3A might be involved in the regulation of TP73-AS1 in osteosarcoma. To our knowledge, this is the first study revealing the role of ARID3A in the regulation of TP73 and TP73-AS1 genes. © 2020 Elsevier Inc.
dc.identifier.doi10.1016/j.genrep.2020.100683
dc.identifier.issn2452-0144
dc.identifier.scopus2-s2.0-85083773591
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1016/j.genrep.2020.100683
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4916
dc.identifier.volume20
dc.identifier.wosWOS:000560609000010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBozgeyik, Esra
dc.language.isoen
dc.publisherElsevier Inc
dc.relation.ispartofGene Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectARID3A
dc.subjectlncRNA
dc.subjectnon-coding RNA
dc.subjectOsteosarcoma
dc.subjectTP73
dc.subjectTP73-AS1
dc.subjectAT rich interaction domain 3a protein
dc.subjectcomplementary RNA
dc.subjectDNA binding protein
dc.subjectsmall interfering RNA
dc.subjecttumor protein p73
dc.subjecttumor protein p73 antisense RNA 1
dc.subjectunclassified drug
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectgene overexpression
dc.subjectgene silencing
dc.subjectosteosarcoma cell
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectprotein expression level
dc.subjectregulatory mechanism
dc.subjectU2OS cell line
dc.titleARID3A-mediated modulation of TP73 and TP73-AS1 in osteosarcoma cells
dc.typeArticle

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