Do major histocompatibility complex tag single nucleotide polymorphisms accurately identify HLA-B27 in the Turkish population?

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dc.authorid0000-0001-7737-4180
dc.authorid0000-0002-3718-171X
dc.authorid0000-0002-8726-3182
dc.authorid0000-0001-9187-3728
dc.authorid0000-0002-1690-3170
dc.authorscopusid7004438602
dc.authorscopusid23473166000
dc.authorscopusid56985556700
dc.authorscopusid11139905400
dc.authorscopusid56052152600
dc.authorscopusid56727893700
dc.authorscopusid57208486786
dc.authorwosidSARI, Ismail/E-1778-2011
dc.authorwosidAkkoc, Nurullah/D-9870-2011
dc.authorwosidBozgeyik, Esra/Z-2966-2019
dc.authorwosidBozgeyik, Esra/AAE-2062-2022
dc.authorwosidIgci, Yusuf Ziya/A-7164-2011
dc.contributor.authorAkar, Servet
dc.contributor.authorİğci, Yusuf Ziya
dc.contributor.authorSarı, İsmail
dc.contributor.authorPala, Elif
dc.contributor.authorGeyik, Esra
dc.contributor.authorTaş, Mehmet Nedim
dc.contributor.authorAkkoç, Nurullah
dc.date.accessioned2022-05-11T14:39:55Z
dc.date.available2022-05-11T14:39:55Z
dc.date.issued2017
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, İç Hastalıkları Ana Bilim Dalı
dc.description.abstractObjectiveTo evaluate the performance of human leukocyte antigen (HLA)-B27 tag single nucleotide polymorphisms (SNPs) by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). MethodsWe genotyped three SNPs (rs116488202, rs13202464 and rs4349859). The primers were designed using Primer3 algorithm via primer-BLAST interface. PCR products were digested by using NlaIII, BmrI and TaqI enzymes. Quality control was performed by DNA sequence analysis. ResultsIn total, 207 patients with ankylosing spondylitis and 32 healthy controls were included in the study. The sensitivity and specificity of SNPs rs116488202 and rs4349859 in identifying HLA-B27 were identical and adequate at 0.946 and 1.000, respectively. On the other hand, the sensitivity and specificity for rs13202464 was 0.878 and 0.934, respectively. The presence of another SNP (rs141774149) in close proximity to rs116488202 complicated the analysis for RFLP and required that we sequence all the T allele carrying samples. ConclusionThe SNPs rs116488202 and rs4349859 may have a place in the identification of HLA-B27 in the Turkish population; however, methods other than PCR-RFLP should be considered.
dc.description.sponsorshipResearch Support Fund of Dokuz Eylul University
dc.description.sponsorshipSource of support in the form of grants or industrial support: this work was supported by the Research Support Fund of Dokuz Eylul University.
dc.identifier.doi10.1111/1756-185X.12719
dc.identifier.endpage2039
dc.identifier.issn1756-1841
dc.identifier.issn1756-185X
dc.identifier.issue12en_US
dc.identifier.pmid26200952
dc.identifier.scopus2-s2.0-84937501514
dc.identifier.scopusqualityQ3
dc.identifier.startpage2035
dc.identifier.urihttps://doi.org/10.1111/1756-185X.12719
dc.identifier.urihttps://hdl.handle.net/20.500.11776/8796
dc.identifier.volume20
dc.identifier.wosWOS:000422713800021
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSolmaz, Dilek
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofInternational Journal of Rheumatic Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectankylosing spondylitis
dc.subjectHLA-B27 antigen
dc.subjectpolymorphism
dc.subjectrestriction fragment length polymorphism
dc.subjectsingle nucleotide polymorphism
dc.subjectAnkylosing-Spondylitis
dc.titleDo major histocompatibility complex tag single nucleotide polymorphisms accurately identify HLA-B27 in the Turkish population?
dc.typeArticle

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