A Novel Biosensing System Using Biological Receptor for Analysis of Vascular Endothelial Growth Factor

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dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorTeke, Mustafa
dc.contributor.authorSayıklı, Çiğdem
dc.contributor.authorCanbaz, Çetin
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:29:52Z
dc.date.available2022-05-11T14:29:52Z
dc.date.issued2014
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractAn immunosensor with rapid and ultrasensitive response for vascular endothelial growth factor (VEGF) has been built up with 4-aminothiophenol (4-ATP) onto the gold surfaces. Quantitative analysis of VEGF was performed by recording the impedance changing of the gold electrode surface by binding of VEGF. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time in the literature. VEGF-R1 was covalently immobilized via 4-ATP self-assembled monolayer formed on gold thin film covered surface. Construction of the biosensor was carefully characterised by the techniques such as electrochemistry and electrochemical impedance spectroscopy. In order to characterize impedance data, Kramers-Kronig transform was performed on the experimental impedance data. The limit of detection of the immunosensor for qualitative detection was 100 pg/mL while the LOD for quantitative detection could down to 100 pg/mL by using the VEGF-R1 based biosensor. Finally, artificial serum samples spiked with VEGF was analyzed by the proposed immunosensor to investigate useful of the biosensor for early biomarker diagnosis.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109 T 172]
dc.description.sponsorshipThe authors greatly appreciate the support of the TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109 T 172).
dc.identifier.doi10.1007/s10989-013-9386-4
dc.identifier.endpage230
dc.identifier.issn1573-3149
dc.identifier.issn1573-3904
dc.identifier.issue2en_US
dc.identifier.startpage221
dc.identifier.urihttps://doi.org/10.1007/s10989-013-9386-4
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7158
dc.identifier.volume20
dc.identifier.wosWOS:000334420100012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.institutionauthorSayıklı, Çiğdem
dc.institutionauthorCanbaz, Çetin
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofInternational Journal of Peptide Research and Therapeutics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectVEGF
dc.subjectCancer biomarker
dc.subjectVEGF-R1
dc.subjectBiosensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSelf-Assembled Monolayers
dc.subjectElectrical Detection
dc.subjectCell Growth
dc.subjectFactor Vegf
dc.subjectImpedance
dc.subjectAngiogenesis
dc.subjectAdsorption
dc.subjectExpression
dc.subjectDispersion
dc.subjectFragments
dc.titleA Novel Biosensing System Using Biological Receptor for Analysis of Vascular Endothelial Growth Factor
dc.typeArticle

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