Comprehensive identification of erythrocyte membrane protein deficiency by 2D gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis

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dc.authorid0000-0002-1798-7951
dc.authorscopusid15842932900
dc.authorscopusid41262197600
dc.authorscopusid6701664192
dc.authorscopusid7102515965
dc.authorwosidTurgut, Burhan/A-2517-2016
dc.authorwosidÖzel Demiralp, Duygu/C-7358-2018
dc.authorwosidAkar, Nejat/H-2949-2019
dc.contributor.authorDemiralp, Duygu Ozel
dc.contributor.authorPeker, Selen
dc.contributor.authorTurgut, Burhan
dc.contributor.authorAkar, Nejat
dc.date.accessioned2022-05-11T14:39:51Z
dc.date.available2022-05-11T14:39:51Z
dc.date.issued2012
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, İç Hastalıkları Ana Bilim Dalı
dc.description.abstractPurpose Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1DE) may reveal qualitative or quantitative defects in red blood cell (RBC) membrane proteins, two-dimensional gel electrophoresis (2DE) can be used for determination of the protein changes caused by the disease process in a relatively high-throughput manner, because it permits an analysis of thousands of modified or unmodified proteins simultaneously. The principal aim of this study was to compare hereditary elliptocytosis (HE), hereditary spherocytosis (HS), and control RBC membrane protein profiles and identify proteins as a clinical marker by the sensitive methods. Experimental design RBC membrane proteins of HE, HS, and control groups were compared by 2DE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS analysis was obtained using peptide mass fingerprint for protein identification. Results Twenty proteins were identified with peptide mass fingerprint analysis and different expression levels were found in HE, HS, and controls for some proteins that includes three biomarker proteins (ankyrin, spectrin, band 3) that may have prognostic or predictive importance. Conclusions and clinical relevance 2DE of RBC proteins is a potentially valuable method for studying heritable disorders such as HE and HS. By identifying a deficiency in membrane proteins associated with the RBC cytoskeleton, the diagnosis of HE and HS can be confirmed.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK).
dc.identifier.doi10.1002/prca.201200010
dc.identifier.endpage411
dc.identifier.issn1862-8346
dc.identifier.issn1862-8354
dc.identifier.issue45511en_US
dc.identifier.pmid22807418
dc.identifier.scopus2-s2.0-84865609351
dc.identifier.scopusqualityQ3
dc.identifier.startpage403
dc.identifier.urihttps://doi.org/10.1002/prca.201200010
dc.identifier.urihttps://hdl.handle.net/20.500.11776/8761
dc.identifier.volume6
dc.identifier.wosWOS:000308093000010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkar, Nejat
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofProteomics Clinical Applications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHereditary elliptocytosis
dc.subjectHereditary spherocytosis
dc.subjectMALDI-TOF MS
dc.subjectTwo-dimensional gel electrophoresis
dc.subjectRed-Cell Membrane
dc.subjectResolution 2-Dimensional Electrophoresis
dc.subjectClinical Expression
dc.subjectSpectrin
dc.subjectMutations
dc.subjectCancer
dc.subjectSeparation
dc.subjectDisorders
dc.subjectDiagnosis
dc.subjectDefects
dc.titleComprehensive identification of erythrocyte membrane protein deficiency by 2D gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis
dc.typeArticle

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