Assessment of UV-vis driven CFT-GO based photocatalysis on the conjugative gene transfer mechanism in a pilot plant system

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dc.contributor.authorÖzkal, Can Burak
dc.date.accessioned2024-10-29T17:50:27Z
dc.date.available2024-10-29T17:50:27Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesien_US
dc.description.abstractThis study addresses the need for sustainable methodologies in antimicrobial resistance (AMR) surveillance, particularly in wastewater treatment, to ensure efficient disinfection and control of AMR. The use of photocatalysis (PC) has gained attention as a scalable and suitable approach for research and development. This study evaluates the effect of UV-vis driven sub-lethal photocatalytic oxidation on conjugative gene transfer between two E. coli strains using a pilot plant reactor system. Photocatalysts composed of graphene-oxide-Ti-CuFe2O4 nanocomposites were synthesized through a green approach and used to enhance bacteria inactivation rates, resulting in hindered frequency and absolute abundance of trans-conjugants in the recipient strains. Experiments plan was built with the intent to determine the contribution of photocatalyst type, mode of operation on the conjugation mechanism and also distinguish between the scenarios where individual or simultaneous exposure to PC oxidation of donor and recipient strains occur. Simultaneous photocatalytic treatment of both donor and recipient strains resulted in the removal of ~3 LOG of both bacteria and eligible conditions were obtained for controlling trans-conjugants formation compared to no treatment conditions. The photocatalyst surface, reactive oxygen species, and bacterial cells' interaction played a determining role in controlling ARG transfer. The impact of photocatalytic oxidation mechanisms on the vitality of recipient cells was evident during the continuous mode of operation, where conjugative transfer of ARGs was mitigated, and the number of trans-conjugants decreased to below 102 CFU mL-1. This study demonstrates the potential of PC for efficient disinfection and control of AMR in wastewater treatment.en_US
dc.identifier.doi10.28948/ngumuh.1285885
dc.identifier.endpage1231en_US
dc.identifier.issn2564-6605
dc.identifier.issue4en_US
dc.identifier.startpage1219en_US
dc.identifier.trdizinid1201909en_US
dc.identifier.urihttps://doi.org/10.28948/ngumuh.1285885
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1201909
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13006
dc.identifier.volume12en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofNiğde Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectPhotocatalysisen_US
dc.subjectAntibiotic resistance geneen_US
dc.subjectConjugative gene transferen_US
dc.subjectBacteria inactivationen_US
dc.titleAssessment of UV-vis driven CFT-GO based photocatalysis on the conjugative gene transfer mechanism in a pilot plant systemen_US
dc.typeArticleen_US

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