Magnetite nanoparticles-based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe

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dc.authorid0000-0001-8347-3783
dc.authorid0000-0002-7754-3527
dc.authorid0000-0003-4688-0552
dc.authorwosidCan, Ziya/D-1919-2019
dc.authorwosidKeskin, Büşra/ABG-1966-2020
dc.authorwosidArda, Ayşem/D-1592-2019
dc.contributor.authorCan, Ziya
dc.contributor.authorKeskin, Büşra
dc.contributor.authorArda, Ayşem
dc.contributor.authorErçağ, Erol
dc.contributor.authorApak, Mustafa Reşat
dc.date.accessioned2022-05-11T14:03:24Z
dc.date.available2022-05-11T14:03:24Z
dc.date.issued2020
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractExcessive amounts of reactive oxygen species (ROS), unless counterbalanced by antioxidants, can cause cellular damage under oxidative stress conditions; therefore, antioxidative defenses against ROS must be measured. With the development of nanotechnology, nanoparticles have found numerous applications in science, health, and industries. Magnetite nanoparticles (Fe3O4:MN Ps) have attracted attention because of their peroxidase-like activity. In this study, hydroxyl radicals (center dot OH) generated by MNPs-catalyzed degradation of H2O2 converted the N,N-dimethyl-p-phenylenediamine (DMPD) probe into its colored DMPD center dot+. radical cation, which gave an absorbance maximum at lambda = 553 nm. In the presence of antioxidants, center dot OH was partly scavenged by antioxidants and produced less DMPD center dot+, causing a decrease in the 553 nm-absorbance. Antioxidant concentrations were calculated with the aid of absorbance differences between the reference and sample solutions. The linear working ranges and trolox equivalent antioxidant capacity coefficients of different classes of antioxidants were determined by applying the developed method. In addition, binary and ternary mixtures of antioxidants were tested to observe the additivity of absorbances of mixture constituents. The method was applied to real samples such as orange juice and green tea. Student t-test, F tests, and the Spearman's rank correlation coefficient were used for statistical comparisons.
dc.description.sponsorshipIstanbul University-Cerrahpasa [28963]
dc.description.sponsorshipThis study was funded by Istanbul University-Cerrahpasa with the project number 28963. We express our gratitude to Istanbul University-Cerrahpasa Application and Research Center for the Measurement of Food Antioxidants (GAAM) for assay development.
dc.identifier.doi10.3906/kim-2006-9
dc.identifier.endpage1375
dc.identifier.issn1300-0527
dc.identifier.issue5en_US
dc.identifier.pmid33488236
dc.identifier.startpage1366
dc.identifier.urihttps://doi.org/10.3906/kim-2006-9
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4692
dc.identifier.volume44
dc.identifier.wosWOS:000582495800013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorErçağ, Erol
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitak
dc.relation.ispartofTurkish Journal Of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMagnetite (Fe3O4) nanoparticles
dc.subjectreactive oxygen species (ROS)
dc.subjectN,N-Dimethyl-p-phenylenediamine
dc.subjectcolorimetric probe
dc.subjectantioxidant activity
dc.subjectAbsorbency Capacity Assay
dc.subjectDietary Polyphenols
dc.subjectOxidative Stress
dc.subjectPower
dc.subjectNeocuproine
dc.subjectSensor
dc.subjectDmpd
dc.titleMagnetite nanoparticles-based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe
dc.typeArticle

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