Label-free and ultrasensitive electrochemical cotinine sensing based on bio-modified magnetic nanoparticles

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dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:29Z
dc.date.available2024-10-29T17:58:29Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractHerein, a label-free impedimetric magneto-biosensor was constructed for detection of cotinine, a promising marker of active and passive smoking. Indium tin oxide sheet (ITO) was utilized as a working electrode and modified magnetic iron oxide (Fe3O4) nanoparticles were attached to the electrode with a neodium magnet. Magnetic iron oxide nanoparticles were modified with a conjugated carboxyl-functionalized poly(pyrrolepropionic acid) (PPPAc) polymer to increase the area and improve the conductivity, which were utilized to immobilize anticotinine antibodies. Anti-cotinine antibodies were specific recognition elements, and a selective sensing platform was constructed by covalent binding of anti-cotinine antibodies. The impedimetric signal was changed with a reaction between cotinine analytes and anti-cotinine antibodies, and the proposed biosensor demonstrated excellent sensitivity, with a wide linear range from 2 to 500 pg/mL and a low detection limit of 0.579 pg/mL. Additionally, this newly constructed magneto-biosensor demonstrated high sensitivity, good reproducibility, and superior specificity. In order to test clinical applicability, serum and saliva samples were analysed with the suggested biosensor, and it indicated excellent performance in comparison with the gold standard methods of enzyme-linked immunoassay (ELISA) and high-performance liquid chromatography (HPLC) systems for the analysis of cotinine. These results displayed that the immunosensor could be potentially useful for clinical diagnostics.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namik Kemal University, Turkey [NKUBAP.00.GA.23.466]
dc.description.sponsorshipFinancial support from the Scientific and Technological Research Council of Tekirdag Namik Kemal University, Turkey (NKUBAP.00.GA.23.466) was gratefully acknowledged.
dc.identifier.doi10.1016/j.snb.2024.135476
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85185881150
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2024.135476
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14349
dc.identifier.volume408
dc.identifier.wosWOS:001192340300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCotinine
dc.subjectMagnetic iron oxide nanoparticles
dc.subjectMagneto-biosensor
dc.subjectLabel-free analysis
dc.titleLabel-free and ultrasensitive electrochemical cotinine sensing based on bio-modified magnetic nanoparticles
dc.typeArticle

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