A New, Sensitive and Disposable Electrochemical Immunosensor Based on Benzaldehyde Side Group Containing Phosphazene Polymer Modified ITO Substrate for Interleukin 1? Detection

Basit Öğe Kaydı
dc.contributor.authorAydın, Muhammet
dc.date.accessioned2022-05-11T14:48:05Z
dc.date.available2022-05-11T14:48:05Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, a novel electrochemical ultrasensitive immunosensor based on disposable benzaldehyde substituted phosp-hazene polymer (BSPP) modified ITO electrode was developed for interleukin 1? (IL 1?) detection. Aldehyde side groups containing phosphazene polymer (BSPP) synthesized via ring opening polymerization method. These aldehyde groups pro-vided anchoring points for anti-IL 1? antibodies. The production process of the proposed immunosensor was monitored by electrochemical techniques like Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV). In addition, these fabrication steps were characterized by utilizing Scanning Electron Microscopy (FE-SEM) and Atomic Force Micros-copy (AFM). Moreover, BSPP polymer layer on the polymer coated electrode surface was investigated by using Energy Dis-persive X-ray (EDX). The fabricated immunosensor had a low detection limit (9.3 fg/mL) and a wide linear detection range (0.03-7.5 pg/mL). Moreover, it had good reproducibility (1.82%), excellent repeatability (1.56%), good selectivity and high stability. The results of experiments showed that the BSPP polymer was desirable platform for IL 1? antigen detection in clinical diagnosis and practical applications. The applicability of the suggested biosensor was tested by measuring IL 1? level in human serum and the suggested immunosensor had acceptable results for quantitative analysis.
dc.identifier.doi10.15671/hjbc.515999
dc.identifier.endpage315
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue3en_US
dc.identifier.startpage305
dc.identifier.trdizinidTXpnMU9UYzBOQT09
dc.identifier.urihttps://doi.org/10.15671/hjbc.515999
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpnMU9UYzBOQT09
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10597
dc.identifier.volume47
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.relation.ispartofHacettepe Journal of Biology and Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA New, Sensitive and Disposable Electrochemical Immunosensor Based on Benzaldehyde Side Group Containing Phosphazene Polymer Modified ITO Substrate for Interleukin 1? Detection
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
10597.pdf
Boyut:
5.4 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu