Potential of flow cytometry in sex determination and in vitro micropropagation of laurus nobilis L.
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Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Corvinus University of Budapest
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Perennial bay laurel (Laurus nobilis L.) is a dioecious economic plant. Its leaves and fruits are considered as important non-wood forest products that are used in food, pharmaceutical, and cosmetic industries. However, it is not possible to distinguish males and females at an early age and this can hinder its multiplication in a planned way. The aim of the present study is to determine the sex of bay laurel at an early stage by analyzing the nuclear DNA (Deoxyribonucleic acid) contents using flow cytometry. The findings of the study showed that the male DNA content is ? 7.95±0.13 pg and the female nuclear DNA content is ? 7.84±0.10 pg. After sex determination, female green axillary buds were cultured on MS (Murashige and Skoog) medium containing different combinations including BAP (6-Benzylaminopurine), NAA (1-Naphthaleneacetic acid) and GA3 (Gibberellic acid) for 42 days to induce micropropagation of the buds. The results indicated 100% shoot regeneration with 2.5±0.85 shoots per explant on MS medium containing 6.6 µM BAP, 5.4 µM NAA, and 0.6 µM GA3. Each randomly selected 45 female shoots were rooted on MS medium containing 4.90 µM IBA (Indole-3-butyric acid) for 12 weeks. Only 16 among them induced roots. However, 13 female plantlets, upon their transfer to pots, showed continuing viability and successful acclimatization to external conditions. Distinguishing sex of bay laurel plants at an early age and establishment of a rapid propagation protocol could offer a significant benefit in breeding and plant production. © 2019, ALÖKI Kft., Budapest, Hungary.
Description
Keywords
Acclimatization, Adult plants, Bay laurel, Dioecious plant, DNA content, Laurus nobilis L., Micropropagation, Sex determination
Journal or Series
Applied Ecology and Environmental Research
WoS Q Value
Scopus Q Value
Q3
Volume
17
Issue
3
