A novel electrochemical impedance immunosensor for the quantification of CYFRA 21-1 in human serum

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:18Z
dc.date.available2024-10-29T17:58:18Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractA sensitive, simple, and reliable immunosensor was constructed to detect the lowest alteration of a fragment of cytokeratin subunit 19 (CYFRA 21-1), a protein lung carcinoma biomarker. The proposed immunosensor was manufactured with a carbon black C45/polythiophene polymer-containing amino terminal groups (C45-PTNH2) conductive nanocomposite, resulting in an excellent, biocompatible, low-cost, and electrically conductive electrode surface. Anti-CYFRA 21-1 biorecognition molecules were attached to the electrode thanks to the amino terminal groups of the used PTNH2 polymer with a relatively simple procedure. All electrode surfaces after modifications were characterized by electrochemical, chemical, and microscopic techniques. Electrochemical impedance spectroscopy (EIS) was also utilized for the evaluation of the analytical feature of the immunosensor. The charge transfer resistance of the immunosensor signal was correlated with the CYFRA 21-1 concentration in the concentration range 0.03 to 90 pg/mL. The limit of detection (LOD) and the limit of quantification (LOQ) of the suggested system were 4.7 fg/mL and 14.1 fg/mL, respectively. The proposed biosensor had favorable repeatability and reproducibility, long storage stability, excellent selectivity, and low cost. Furthermore, it was applied to determine CYFRA 21-1 in commercial serum samples, and satisfactory recovery results (98.63-106.18%) were obtained. Thus, this immunosensor can be offered for clinical purposes as a rapid, stable, low-cost, selective, reproducible, and reusable tool.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namik Kemal University, Tekirdag, Turkey [NKUBAP.00.GA.21.328]
dc.description.sponsorshipThe authors acknowledge the Scientific and Technological Research Council of Tekirdag Namik Kemal University for the funding of this work through project NKUBAP.00.GA.21.328, Tekirdag, Turkey.
dc.identifier.doi10.1007/s00604-023-05813-z
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue6en_US
dc.identifier.pmid37219635
dc.identifier.scopus2-s2.0-85160019549
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-023-05813-z
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14207
dc.identifier.volume190
dc.identifier.wosWOS:000994249100002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarbon black C45
dc.subjectConjugated polymer
dc.subjectLabel-free detection
dc.subjectDisposable immunosensor
dc.subjectIndium tin oxide electrode
dc.subjectSurface modified electrode
dc.titleA novel electrochemical impedance immunosensor for the quantification of CYFRA 21-1 in human serum
dc.typeArticle

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