Electrochemical immunosensor based on chitosan/conductive carbon black composite modified disposable ITO electrode: An analytical platform for p53 detection

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dc.authorid0000-0002-8716-9970
dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.authorwosidAYDIN, MUHAMMET/ABA-9027-2020
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:14Z
dc.date.available2022-05-11T14:48:14Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, we fabricated a label-free electrochemical immunosensor for sensitive and selective detection of tumor marker p53. This immunosensor was based on chitosan/carbon black composite (Chitosan-CB) layer coated ITO electrode. This composite was utilized for enhancement of the conductivity of the immunosensor. Anti-p53 antibodies were captured on the modified ITO electrode through the cross-linking of chitosan and glutaraldehyde. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were utilized for electrochemical characterization of the proposed immunosensor. Moreover, the biosensor construction steps were monitored by using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The immobilization of anti-p53 antibodies on the electrode surface was investigated by using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The change in impedance which formed during the specific interaction between anti-p53 antibody and p53 antigen was used to detect p53. Under optimized experimental conditions, the fabricated immunosensor had a wide linear range of 0.01-2 pg/mL and low detection limit of 3 fg/mL. The fabricated immunosensor had good sensitivity, stability and repeatability. Furthermore, it was successfully applied to analyze p53 in human serum.
dc.description.sponsorshipScientific and Technological Research Council of by Tekirdag Namik Kemal University, NKU-BAP, Turkey [NKUBAP.00.DS.17.114]
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirdag Namik Kemal University, NKU-BAP, Turkey (Grant number: NKUBAP.00.DS.17.114).
dc.identifier.doi10.1016/j.bios.2018.09.008
dc.identifier.endpage89
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid30199712
dc.identifier.scopus2-s2.0-85052963153
dc.identifier.scopusqualityQ1
dc.identifier.startpage80
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.09.008
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10615
dc.identifier.volume121
dc.identifier.wosWOS:000447819700011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectChitosan
dc.subjectp53
dc.subjectConductive carbon black
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSuppressor Protein P53
dc.subjectHuman Serum
dc.subjectLabel-Free
dc.subjectLow-Cost
dc.subjectChitosan
dc.subjectCancer
dc.subjectBiomarker
dc.subjectPolymer
dc.subjectImmobilization
dc.subjectBiosensor
dc.titleElectrochemical immunosensor based on chitosan/conductive carbon black composite modified disposable ITO electrode: An analytical platform for p53 detection
dc.typeArticle

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