Astragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.

dc.authorid0000-0003-1262-063X
dc.authorid0000-0002-7002-9752
dc.authorid0000-0002-9078-2186
dc.authorscopusid22835706400
dc.authorscopusid7006520055
dc.authorscopusid26648037000
dc.authorscopusid7003998497
dc.authorwosidBedir, Erdal/ABI-3895-2020
dc.authorwosidGurel, Aynur/AAG-8700-2019
dc.contributor.authorNartop, Pınar
dc.contributor.authorGürel, Aynur
dc.contributor.authorAkgün, İsmail Hakki
dc.contributor.authorBedir, Erdal
dc.date.accessioned2022-05-11T14:16:01Z
dc.date.available2022-05-11T14:16:01Z
dc.date.issued2015
dc.departmentFakülteler, Çorlu Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractIn the present paper, an efficient method for clonal propagation of an endemic plant, Astragalus trojanus Stev., was described. Effect of sucrose and 6-benzyladenine (BA) concentrations on shoot proliferation and rooting was studied. Woody plant medium (WPM) supplemented with two different concentrations of BA (S1 medium with 0.5 mg/L BA & S2 medium with 1 mg/L BA) was used, where S2 medium resulted in higher rates in both shoot elongation and multiple shoot proliferation of clonally propagated A. trojanus shoot cultures. Of 27 clones, 3 were selected for further studies based on the highest regeneration capacity (Clones 1, 12 & 15). A total of 340 shoots were obtained from Clone 1 in S1 medium. In this medium, the rooting ratio of shoots for Clone 15 was 11.67%. The highest shoot elongation (1.99 cm), multiple shoot proliferation ratio (4.35) and multiple shoot proliferation percentage (91.67%) were obtained from Clone 1 in S2 medium. Two media, WPM medium supplemented with 15 g/L (K1 medium) and 30 g/L (K2 medium) sucrose were used for rooting. The root formation of Clone 1 was 26.67% in K2 medium. The highest astragaloside IV (0.5486 mu g/mg) and cycloastragenol (0.1263 mu g/mg) contents were detected in roots grown in K1 medium.
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-109O627]
dc.description.sponsorshipThe financial support of The Scientific and Technical Research Council of Turkey (TUBITAK) (Project No. TOVAG-109O627) is gratefully acknowledged.
dc.identifier.endpage546
dc.identifier.issn0972-5849
dc.identifier.issn0975-0967
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84962220495
dc.identifier.scopusqualityQ4
dc.identifier.startpage540
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6153
dc.identifier.volume14
dc.identifier.wosWOS:000375820000014
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorNartop, Pınar
dc.language.isoen
dc.publisherNatl Inst Science Communication-Niscair
dc.relation.ispartofIndian Journal of Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAstragalus trojanus
dc.subjectastragaloside IV
dc.subjectcycloastragenol
dc.subjectmultiple shoot proliferation
dc.subjectrooting
dc.subjectCycloartane Triterpene Glycosides
dc.subjectSecondary Metabolites
dc.subjectSomatic Embryogenesis
dc.subjectPlant-Regeneration
dc.subjectCultures
dc.subjectRoots
dc.subjectMicropropagation
dc.subjectSucrose
dc.titleAstragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.
dc.typeArticle

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