Effect of PDE 5 Inhibitor-Avanafil on Renal Ischemia/Reperfusion Injury in Rats

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dc.authoridbozkurt, ayse/0000-0003-1551-949X
dc.authoridCivelek, Maide Sena/0000-0001-8621-2765
dc.authoridTAVACI, Taha/0000-0002-8284-8317
dc.authoridHalici, Zekai/0000-0001-6854-6059
dc.authoridOzdemir Sarikaya, Bengul/0000-0003-2642-6096
dc.contributor.authorYuksel, Tugba Nurcan
dc.contributor.authorHalici, Zekai
dc.contributor.authorKaya, Cihangir
dc.contributor.authorBozkurt, Ayse
dc.contributor.authorTavaci, Taha
dc.contributor.authorCivelek, Maide Sena
dc.contributor.authorOzdemir, Bengul
dc.date.accessioned2024-10-29T17:59:37Z
dc.date.available2024-10-29T17:59:37Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractAim: Renal ischemia-reperfusion injury (RI/RI) damages many organs, especially the kidney. Phosphodiesterase (PDE) 5 inhibitors has antioxidant and anti-inflammatory effects. Avanafil (AVA) is a second-generation PDE 5 inhibitor with greater PDE isoform selectivity. The aim of this study is to investigate the effects of AVA on RI/RI in rats. Materials and Methods: Forty rats were randomly divided into five groups (n=8): Sham; AVA 10; RI/RI; RI/RI + 5 mg/kg AVA, and RI/RI + 10 mg/ kg AVA. RI/RI in rats was established by clamping renal artery. An acute surgical experiment was performed for the induction of renal ischemia for 45 min by renal artery clamping followed by reperfusion for 24 h. Kidney tissues were investigated biochemically [malondialdehyde (MDA) and glutathione (GSH) with ELISA], molecularly [relative quantification of IL-113, nuclear factor-kappa B (NF-KB), and tumor necrosis factor-alpha (TNF-a) mRNA gene expression with qRT-PCR], and histopathologically (staining with Harris hematoxylin and eosin Y). Results: AVA administration ameliorated disturbances in MDA and GSH levels caused by RI/RI. AVA treatment improved the increase in the mRNA expressions of IL-113, NF-KB, and TNF-a in kidney tissues induced ischemia/reperfusion injury. AVA administration ameliorated histopathologic injury in kidney tissues caused by renal ischemia reperfusion. Moreover, the values closest to those of the sham group were obtained by administering 10 Conclusion: AVA administration improved renal ischemia/reperfusion-induced tissue injury by alleviating oxidative stress and inflammatory cascades that could be important in ischemia-reperfusion injury. These findings may provide a mechanistic basis for using AVA to treat RI/RI.
dc.identifier.doi10.4274/nkmj.galenos.2023.74436
dc.identifier.endpage293
dc.identifier.issn2587-0262
dc.identifier.issue3en_US
dc.identifier.startpage284
dc.identifier.trdizinid#BAŞV!
dc.identifier.urihttps://doi.org/10.4274/nkmj.galenos.2023.74436
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1263215
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14787
dc.identifier.volume11
dc.identifier.wosWOS:001187553300008
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherGalenos Publ House
dc.relation.ispartofNamik Kemal Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnti-inflammatory
dc.subjectantioxidant
dc.subjectavanafil
dc.subjectphosphodiesterase 5 inhibitor
dc.subjectrenal ischemia/reperfusion injury
dc.titleEffect of PDE 5 Inhibitor-Avanafil on Renal Ischemia/Reperfusion Injury in Rats
dc.title.alternativeSıçanlarda Renal İskemi/Reperfüzyon Hasarı Üzerine PDE 5 İnhibitörü-Avanafilin Etkisi
dc.typeArticle

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