A label-free and sensitive impedimetric immunosensor for TNF ? biomarker detection based on epoxysilane-modified disposable ITO-PET electrode

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dc.authorscopusid57194273220
dc.contributor.authorBurcu Aydın, E.
dc.date.accessioned2022-05-11T14:48:18Z
dc.date.available2022-05-11T14:48:18Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractThe present study constructed a simple and cost-effective electrochemical immunosensor based on 3-glycidoxypropyltriethoxysilane (GPTES) monolayer coated indium tin oxide-polyethylene terephthalate (ITO-PET) electrode for selective and sensitive determination of Tumour necrosis factor ? (TNF ?) cancer biomarker. GPTES was a well-known surface coupling silane agent which had epoxy groups to bind antibodies. Anti-TNF ? antibodies were chosen as biorecognition elements and they attached covalently on the GPTES modified ITO-PET electrode. Electrochemical determination of immunoreaction between anti-TNF ? antibodies and TNF ? antigens was the basic principle of the proposed immunosensor. The electrochemical characterisation of the designed biosensor was tested with electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. In addition, the surface morphological characteristics of the ITO-PET electrodes after several modification stages were monitored by scanning electron microscopy (SEM) and atomic force microscopy (AFM) imaging. The analytical success of the proposed immunosensor was tested with respect to linear detection range, detection limit, repeatability, reproducibility, reusability, regeneration and long-term stability. The present biosensor had a wide linear range from 0.01 to 1.5 pg/mL and a low detection limit of 3.1 fg/mL. Finally, the biosensor successfully detected TNF ? cancer biomarker in human serum samples with high recovery ranges. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.doi10.1080/03067319.2019.1679807
dc.identifier.endpage377
dc.identifier.issn0306-7319
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85074584482
dc.identifier.scopusqualityQ3
dc.identifier.startpage363
dc.identifier.urihttps://doi.org/10.1080/03067319.2019.1679807
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10623
dc.identifier.volume100
dc.indekslendigikaynakScopus
dc.institutionauthorBurcu Aydın, E.
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofInternational Journal of Environmental Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject3-glycidoxypropyl7triethoxysilane
dc.subjectelectrochemical impedance spectroscopy
dc.subjectindium tin oxide-polyethylene terephthalate (ITO-PET) electrode
dc.subjectTumour necrosis factor ?
dc.subjectAntibodies
dc.subjectAtomic force microscopy
dc.subjectBiomarkers
dc.subjectCell death
dc.subjectChemical detection
dc.subjectCost effectiveness
dc.subjectCyclic voltammetry
dc.subjectDiseases
dc.subjectElectrochemical impedance spectroscopy
dc.subjectGlycoproteins
dc.subjectImmunosensors
dc.subjectMacrophages
dc.subjectPlastic bottles
dc.subjectPolyethylene terephthalates
dc.subjectReusability
dc.subjectScanning electron microscopy
dc.subjectSpectroscopy
dc.subjectTin oxides
dc.subjectTumors
dc.subject3-glycidoxypropyl7triethoxysilane
dc.subjectElectrochemical determination
dc.subjectElectrochemical immunosensors
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide
dc.subjectMorphological characteristic
dc.subjectNecrosis factors
dc.subjectTumour necrosis factor alphas
dc.subjectElectrochemical electrodes
dc.titleA label-free and sensitive impedimetric immunosensor for TNF ? biomarker detection based on epoxysilane-modified disposable ITO-PET electrode
dc.typeArticle

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