DNA repair and apoptosis: Roles in radiotherapy-related acute reactions in breast cancer patients

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dc.authorid0000-0002-5300-4449
dc.authorid0000-0002-8749-1708
dc.authorid0000-0003-3601-6074
dc.authorscopusid15764510600
dc.authorscopusid56921991100
dc.authorscopusid56688626600
dc.authorscopusid57211121669
dc.authorscopusid6506076713
dc.authorscopusid6602311398
dc.authorscopusid56742898900
dc.authorwosidTuncdemir, Matem/P-3054-2018
dc.authorwosidGUVEN, MEHMET/C-9833-2019
dc.contributor.authorBatar, Bahadır
dc.contributor.authorMutlu, Tuba
dc.contributor.authorBostanci, Merve
dc.contributor.authorAkın, Mustafa
dc.contributor.authorTuncdemir, Matem
dc.contributor.authorBese, Nuran
dc.contributor.authorGüven, Mehmet
dc.date.accessioned2022-05-11T14:42:07Z
dc.date.available2022-05-11T14:42:07Z
dc.date.issued2018
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractNormal tissue reactions are therapy limiting factor for the effectiveness of the radiotherapy in cancer patients. DNA repair and apoptosis are estimated to be critical players of adverse effects in response to radiotherapy. Our aim was to define the association of DNA repair (ERCC1 and XPC) and apoptotic (BCL2, CASP3 and NFKB1) gene expression, DNA damage levels, apoptosis changes and DNA repair gene variations with the risk of acute side effects in breast cancer patients. The study included 100 women with newly diagnosed breast cancer; an experimental case group (n=50) with acute side effects and the control group (n=50) without side effects. Gene expression was analyzed by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). Micronucleus (MN) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) assays were performed to compare the DNA damage levels. Apoptosis was examined by TDT-mediated dUTP-biotin nick end-labeling (TUNEL) staining. ERCC1 rs3212986 and XPC rs3731055 polymorphisms were genotyped by real-time PCR technique. No significantly correlation of DNA repair and apoptosis gene expression and DNA damage levels with acute side effects in response to radiotherapy. Also, there was no association between apoptosis levels and acute effects. ERCC1 rs3212986 CC genotype showed a protective effect against radiotherapy-induced acute reactions (p<0.001; OR: 0.21; 95% CI=0.08-0.52). Our results suggest that apoptosis and DNA damage levels are not associated with acute radiosensitivity. DNA repair may affect the risk of acute reactions. Further studies are needed to validate the current findings.
dc.description.sponsorshipResearch Fund of The University of IstanbulIstanbul University [25182]
dc.description.sponsorshipThe study was funded by Research Fund of The University of Istanbul (25182).
dc.identifier.doi10.14715/cmb/2018.64.4.11
dc.identifier.endpage70
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue4en_US
dc.identifier.pmid29631685
dc.identifier.scopus2-s2.0-85044718742
dc.identifier.scopusqualityQ4
dc.identifier.startpage64
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.4.11
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9234
dc.identifier.volume64
dc.identifier.wosWOS:000429488200011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBatar, Bahadır
dc.language.isoen
dc.publisherC M B Assoc
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcute side effects
dc.subjectApoptosis
dc.subjectBreast cancer
dc.subjectDNA damage
dc.subjectDNA repair
dc.subjectERCC1
dc.subjectRadiotherapy
dc.subjectPeripheral-Blood Lymphocytes
dc.subjectNucleotide Excision-Repair
dc.subjectChromosomal Radiosensitivity
dc.subjectRadiation-Therapy
dc.subjectMessenger-Rna
dc.subjectLate Toxicity
dc.subjectDamage
dc.subjectExpression
dc.subjectPolymorphisms
dc.subjectGenes
dc.titleDNA repair and apoptosis: Roles in radiotherapy-related acute reactions in breast cancer patients
dc.typeArticle

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