Bacteroidetes and Firmicutes levels in gut microbiota and effects of hosts TLR2/TLR4 gene expression levels in adult type 1 diabetes patients in Istanbul, Turkey

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dc.authorid0000-0001-9670-2426
dc.authorid0000-0002-2205-5120
dc.authorid0000-0003-1072-3846
dc.authorscopusid57220204043
dc.authorscopusid56246709000
dc.authorscopusid57194164764
dc.authorscopusid56118245000
dc.authorscopusid6507755715
dc.authorscopusid57211552167
dc.authorscopusid6506930479
dc.authorwosidDemirci, Mehmet/O-1886-2015
dc.authorwosidKiraz, Nuri/AAI-9380-2021
dc.authorwosidTokman, Hrisi Bahar/Y-2514-2018
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorTokman, Hrisi Bahar
dc.contributor.authorTaner, Zeynep
dc.contributor.authorKeskin, Fatma Ela
dc.contributor.authorÇağatay, Penbe
dc.contributor.authorBakar, Yesim Ozturk
dc.contributor.authorKocazeybek, Bekir S.
dc.contributor.authorKiraz, Nuri
dc.date.accessioned2022-05-11T14:42:11Z
dc.date.available2022-05-11T14:42:11Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractAim: The aim of this study was to determine and compare the levels of both Bacteroidetes and Firmicutes in the gut microbiota and TLR2/TLR4 gene expression in the blood of patients with type 1 diabetes mellitus (T1DM) and healthy individuals. These results may serve as a preliminary assessment to guide future research. Method: Between January and October 2014, stool and blood samples were collected from 53 adult T1DM patients and 53 age- and gender-matched healthy individuals. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and TLR2 and TLR4 expression levels were analyzed via qPCR using RNA from EDTA blood samples from both patients and healthy controls. Results: The amounts of Bacteroidetes and Firmicutes were statistically significantly higher and lower, respectively, in the T1DM group than in the healthy control group (p < 0.001 and p < 0.001, respectively). In addition, the Firmicutes/Bacteroidetes ratios in patients with T1DM were significantly lower than in healthy controls. The TLR4 and TLR2 gene expression levels in T1DM patients were significantly upregulated and downregulated, respectively, compared to those in the control group. Conclusion: Our data are the first to show a relationship between T1DM and gut microbiota in our country. In addition, our results provide information about the connections between T1DM, gut microbiota, and TLR2 and TLR4 expression. We believe that Bacteroidetes and Firmicutes in the gut microbiota may play a role in the autoimmune process of T1DM and that these findings should be further investigated in the future. (C) 2019 Elsevier Inc All rights reserved.
dc.description.sponsorshipScientific Research Projects Unit of Istanbul UniversityIstanbul University [37749]
dc.description.sponsorshipThis research was supported by Scientific Research Projects Unit of Istanbul University. Project No: 37749.
dc.identifier.doi10.1016/j.jdiacomp.2019.107449
dc.identifier.issn1056-8727
dc.identifier.issn1873-460X
dc.identifier.issue2en_US
dc.identifier.pmid31677982
dc.identifier.scopus2-s2.0-85074431457
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.jdiacomp.2019.107449
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9268
dc.identifier.volume34
dc.identifier.wosWOS:000512832000010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKiraz, Nuri
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofJournal of Diabetes and Its Complications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectType 1 diabetes
dc.subjectBacteroidetes
dc.subjectFirmicutes
dc.subjectGut microbiota
dc.subjectToll-like receptors
dc.subjectIntestinal Microbiota
dc.subjectQuantitative Pcr
dc.subjectFecal Microbiota
dc.subjectChildren
dc.subjectBlood
dc.subjectTlr4
dc.titleBacteroidetes and Firmicutes levels in gut microbiota and effects of hosts TLR2/TLR4 gene expression levels in adult type 1 diabetes patients in Istanbul, Turkey
dc.typeArticle

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