A novel designed chitosan based hydrogelwhich is capable of consecutively controlled release of TGF-Beta 1 and BMP-7

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dc.authorscopusid35197435300
dc.authorscopusid37095337000
dc.authorscopusid14622670700
dc.authorscopusid8450175300
dc.authorscopusid57197122663
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorGökay, Nevzat Selim
dc.contributor.authorGökçe, Alper
dc.contributor.authorTonbul, Murat
dc.contributor.authorGökçe, Çiğdem
dc.date.accessioned2022-05-11T14:36:08Z
dc.date.available2022-05-11T14:36:08Z
dc.date.issued2013
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dal
dc.description.abstractObjective: Both Bone Morphogenetic Protein 7 (BMP-7) and Transforming Growth Factor Beta 1 (TGF-ß1) have stimulating effects on chondrocyte proliferation. These growth factors act at different time points of the biological repair process. We aimed to design a hydrogel, which would enable consecutive controlled release of the growth factors. Material and Methods: We designed a chitosan particle impregnated Poly (vinylalcohol) (PVA)-borax hydrogel (CPBH). CPBH allowed controlled release of BMP-7 and TGF-ß1 at different time points. Hydrogel structure was analyzed by Scanning Electron Microscope (SEM). The release kinetics of Growth Factors (GFs) were determined with enzyme-linked immunosorbent assay (ELISA) and UV-spectrophotometer. Chondrocyte viability and toxicity were tested through CellTiter 96® Non-Radioactive Cell Proliferation (MTS) ELISA. Results: The designed hydrogel showed high swelling and mucoadhesion characteristics under acidic conditions. CPBH released BMP-7 first and rapidly and TGF-ß1 consecutively and slowly. It also allowed controlled release of protein/peptide based drugs for 21 days without altering their bioactive properties. At the end of 21 days, 82.62% of BMP-7 and 98.34% of TGF-ß1 were consecutively released. The difference between the groups was significant (for TGF-ß1, p<0.05 and for BMP-7, p<0.001). Conclusion: The controlled and slow release of growth factors has been shown to be beneficial on cartilage regeneration. As it is not cytotoxic, we suggest that this hydrogel might be used in medical and pharmaceutical applications areas. © 2013 by Türkiye Klinikleri.
dc.identifier.doi10.5336/medsci.2011-27807
dc.identifier.endpage32
dc.identifier.issn1300-0292
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84872084572
dc.identifier.scopusqualityQ4
dc.identifier.startpage18
dc.identifier.urihttps://doi.org/10.5336/medsci.2011-27807
dc.identifier.urihttps://hdl.handle.net/20.500.11776/8382
dc.identifier.volume33
dc.indekslendigikaynakScopus
dc.institutionauthorYılmaz, İbrahim
dc.institutionauthorGökay, Nevzat Selim
dc.institutionauthorGökçe, Alper
dc.institutionauthorTonbul, Murat
dc.language.isoen
dc.publisherTurkiye Klinikleri
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBone morphogenetic protein 7
dc.subjectDelayed-action preparations
dc.subjectHuman
dc.subjectHydrogels
dc.subjectTGF-ß1 protein
dc.subjectchitosan
dc.subjectosteogenic protein 1
dc.subjectpolymer
dc.subjecttransforming growth factor beta1
dc.subjectabsorption
dc.subjectacidity
dc.subjectadult
dc.subjectarticle
dc.subjectbiodegradation
dc.subjectcartilage cell
dc.subjectcase report
dc.subjectcell growth
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcontrolled drug release
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjecthydrogel
dc.subjecthydrophilicity
dc.subjecthydrophobicity
dc.subjectparticle size
dc.subjectpH
dc.subjectprotein secretion
dc.subjectsynthesis
dc.subjecttemperature
dc.subjectultraviolet spectroscopy
dc.titleA novel designed chitosan based hydrogelwhich is capable of consecutively controlled release of TGF-Beta 1 and BMP-7
dc.title.alternativeTGF-Beta 1 ve BMP-7'nin ardisik ve kontrollü salimina izin veren yeni kitosan bazli bir hidrojel dizayni]
dc.typeArticle

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