Amperometric biosensor for xanthine determination based on Fe 3O4 nanoparticles

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dc.contributor.authorÖztürk, Funda Özcan
dc.contributor.authorErden, Pinar Esra
dc.contributor.authorKaçar, Ceren
dc.contributor.authorKiliç, Esma
dc.date.accessioned2024-10-29T17:43:45Z
dc.date.available2024-10-29T17:43:45Z
dc.date.issued2014
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractAn amperometric xanthine biosensor was developed based on the immobilization of xanthine oxidase (XO) into the Fe3O4 nanoparticles modified carbon paste. Electron transfer properties of unmodified and Fe3O4 nanoparticles modified carbon paste electrodes were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Fe3O4 nanoparticles increased electroactive surface area of the electrode and electron transfer at solution/electrode interface. Optimum pH, nanoparticle loading and enzyme loading were found to be 6.0; 14.2% and 0.6 Unit XO respectively. Fe3O4 nanoparticles modified carbon paste enzyme electrode allowed xanthine determination at -0.20 V, thus minimizing the potential interferences from electrochemically oxidizable substances such as ascorbic acid and uric acid. A linear relationship was obtained in the concentration range from 7.4 × 10-7 mol L-1 to 7.5 × 10-5 mol L -1 and a detection limit of 2.0 × 10-7 mol L -1. The biosensor was used for determination of xanthine in urine samples and the results indicate that the biosensor is effective for the detection of xanthine.
dc.identifier.endpage26
dc.identifier.issn1318-0207
dc.identifier.issue1en_US
dc.identifier.pmid24664322
dc.identifier.scopus2-s2.0-84897554722
dc.identifier.scopusqualityQ3
dc.identifier.startpage19
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12603
dc.identifier.volume61
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSlovensko Kemijsko Drustvo
dc.relation.ispartofActa Chimica Slovenica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAmperometry
dc.subjectBiosensor
dc.subjectCarbon paste
dc.subjectXanthine
dc.titleAmperometric biosensor for xanthine determination based on Fe 3O4 nanoparticles
dc.typeArticle

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