Novel electrochemical biosensing platform based on conductive multilayer for sensitive and selective detection of CYFRA 21-1

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dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-05-06T17:23:45Z
dc.date.available2023-05-06T17:23:45Z
dc.date.issued2023
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn the present work, an electrochemical biosensor modified with a conductive multilayer composed of gold nanoparticles and amino-substituted-pyrrole polymer (AuNPs/P(PyAmn)) was constructed for label-free detec-tion of cytokeratin 19 fragment 21-1 (CYFRA 21-1), one of the most important lung cancer biomarkers. The electrochemical biosensor was fabricated by electropolymerization of PyAmn on an indium tin oxide (ITO) platform with electrodeposition of AuNPs and binding of an anti-CYFRA 21-1 recognition element on the P (PyAmn)-coated electrode via glutaraldehyde crosslinking. In the present immunosensor, AuNPs deposition provided excellent conductivity and good electron transfer. The electropolymerization of the P(PyAmn) polymer layer ensured a large surface area that increased the amount of anti-CYFRA 21-1 antibodies that could be attached. A specific immunoreaction occurred between anti-CYFRA 21-1 antibody and CYFRA 21-1 antigen when the immunosensor was successively reacted with the target CYFRA 21-1 protein. According to specific immu-noreactions, the resulting biosensor produced an impedimetric signal proportional to the amount of CYFRA 21-1 in the linear detection range of 0.015-90 pg/mL. With optimal fabrication parameters, the detection limit, quantification limit and sensitivity for CYFRA 21-1 were 4.59 pg/mL, 15.31 pg/mL and 0.21 k Omega pg(-1) mL cm(-2), respectively. Meanwhile, this biosensor had good stability and high specificity for CYFRA 21-1 detection, and it was successfully applied to real serum samples. It also had a satisfactory rate of R-2 = 0.9984. The use of a disposable ITO substrate as a basis for this immunosensor allowed the use of simple instrumentation. This study illustrates a new approach to fabricate an ultrasensitive and label-free electrochemical biosensor based on a disposable platform for CYFRA 21-1 analysis in clinical biosensing.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag ?; [NKU- BAP.00]
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Tekirdag ? Nam?k Kemal University, (Project number: NKU- BAP.00.GA.21.328) .
dc.identifier.doi10.1016/j.snb.2022.133208
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85144418835
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2022.133208
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12290
dc.identifier.volume378
dc.identifier.wosWOS:000904641700003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors And Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCytokeratin 19 fragment 21-1
dc.subjectConducting polymer
dc.subjectLabel-less biosensor
dc.subjectLung cancer
dc.subjectLung-Cancer
dc.subjectImmunosensor
dc.subjectPolymer
dc.subjectPolythiophene
dc.subjectElectrode
dc.subjectGraphene
dc.subjectSerum
dc.titleNovel electrochemical biosensing platform based on conductive multilayer for sensitive and selective detection of CYFRA 21-1
dc.typeArticle

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