Genista januensis subsp. lydia (fabaceae) bitkisinin biyoaktivite rehberli izolasyonu ve antikanser, antioksidan aktivitelerinin araştırılması
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tekirdağ Namık Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Genista januensis subsp. lydia (Boiss.) Kit Tan & Ziel. bitkisinin antikanser ve antioksidan aktivitelerinin incelenmesi, aktiviteden sorumlu fitokimyasal bileşiklerin, özellikle yeni fenolik/flavonoid bileşiklerinin izolasyonu, kimyasal yapılarının belirlenmesi, uçucu bileşiklerinin tayini ve miktarı yeterli olan saf bileşiklerde antikanser ve antioksidan aktivitelerinin incelenmesi amaçlanmıştır. Çalışma kapsamında G. januensis bitkisinin; hekzan, kloroform, etil asetat, metanol çözücüleri kullanılarak, maserasyon yöntemiyle ekstraksiyonu yapılmış ve ham ekstrelerde sitotoksisite, apoptoz, hücre döngüsü analizleri yapılarak antikanser etkisi araştırılmıştır. Ayrıca ham ekstrelerde toplam fenolik/flavonoid madde miktarı tayini ile DPPH, ABTS ve FRAP yöntemleri kullanılarak antioksidan aktiviteleri belirlenmiştir. Elde edilen ekstrelerin antikanser ve antioksidan aktivite sonuçlarına göre en yüksek aktivite gösteren ekstrenin, etil asetat ekstresi olduğu belirlenmiş ve izolasyon çalışması için etil asetat ekstresi seçilmiştir. Etil asetat ekstresinin kromatografik yöntemlerle izolasyonu yapılmış ve izole edilen bileşiklerin 1D, 2D NMR ve kütle spektroskopisi ile molekül yapıları aydınlatılmıştır. Çalışmanın sonucunda, G. januensis bitkisinin etil asetat ekstresinden 3 izoflavonoid, 1 biflavonoid, 1 fenolik bileşik ve 2 şeker türevi olan 7 bileşik izole edilmiştir. İzole edilen 7 bileşik G. januensis bitkisinden ilk defa izole edilmiştir. Miktarı yeterli olan izole bileşiklerin sitotoksisitesi araştırılmıştır. (5), (6) ve (7) numaralı bileşiklerin antikanser etkiye sahip olduğu düşünülmektedir. Bununla birlikte; (4) numaralı antioksidan aktivitesi araştırılmıştır.
This study was designed to examine the anticancer and antioxidant activities of the Genista januensis subsp. lydia (Boiss.) Kit Tan & Ziel. in crude extracts, isolated phenolic/flavonoid compounds which responsible for the biological activities. Isolation of phytochemical compounds, and determination of their chemical structures aimed in this study along with investigate of their bioactivity. Additionally, volatile compounds were determined by GC-MS method in hexane extract. Within this scope of study, extraction of G. januensis plant was done by maceration method using hexane, chloroform, ethyl acetate and methanol solvents and their anticancer effect was investigated by performing cytotoxicity, apoptosis, and cell cycle analyses in crude extracts. In addition to total phenolic/flavonoid content, ABTS, DPPH, FRAP activity determined in raw extracts. According to the results of anticancer and antioxidant activity in crude extracts, ethyl acetate extract, which has the highest activity in all extracts, was selected for isolation study. Ethyl acetate extract was used for isolation by chromatographic methods and isolated compounds were elucidated by 1D and 2D NMR and mass spectroscopy methods for determination their molecular structures. As a result of this study, 7 compounds, which were 3 isoflavonoids, 1 biflavonoid, 1 phenolic compound and 2 sugar derivatives were isolated from the ethyl acetate extract of the G. januensis plant. Cytotoxicity tests were done for sufficient amount of isolated compounds in anticancer studies. (5), (6) and (7) numbered compounds are thought to have anticarcinogenic effects. The antioxidant activity determination only was performed on (4) numbered compound because of the insufficient amounts of other isolated compounds.
This study was designed to examine the anticancer and antioxidant activities of the Genista januensis subsp. lydia (Boiss.) Kit Tan & Ziel. in crude extracts, isolated phenolic/flavonoid compounds which responsible for the biological activities. Isolation of phytochemical compounds, and determination of their chemical structures aimed in this study along with investigate of their bioactivity. Additionally, volatile compounds were determined by GC-MS method in hexane extract. Within this scope of study, extraction of G. januensis plant was done by maceration method using hexane, chloroform, ethyl acetate and methanol solvents and their anticancer effect was investigated by performing cytotoxicity, apoptosis, and cell cycle analyses in crude extracts. In addition to total phenolic/flavonoid content, ABTS, DPPH, FRAP activity determined in raw extracts. According to the results of anticancer and antioxidant activity in crude extracts, ethyl acetate extract, which has the highest activity in all extracts, was selected for isolation study. Ethyl acetate extract was used for isolation by chromatographic methods and isolated compounds were elucidated by 1D and 2D NMR and mass spectroscopy methods for determination their molecular structures. As a result of this study, 7 compounds, which were 3 isoflavonoids, 1 biflavonoid, 1 phenolic compound and 2 sugar derivatives were isolated from the ethyl acetate extract of the G. januensis plant. Cytotoxicity tests were done for sufficient amount of isolated compounds in anticancer studies. (5), (6) and (7) numbered compounds are thought to have anticarcinogenic effects. The antioxidant activity determination only was performed on (4) numbered compound because of the insufficient amounts of other isolated compounds.
Açıklama
Fen Bilimleri Enstitüsü, Kimya Ana Bilim Dalı, Organik Kimya Bilim Dalı
Anahtar Kelimeler
Kimya, Chemistry
