First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques

Basit Öğe Kaydı
dc.contributor.authorYücel, Gülru
dc.contributor.authorKolano, Bozena
dc.contributor.authorCabi, Evren
dc.contributor.authorTuna, Metin
dc.date.accessioned2024-10-29T17:50:28Z
dc.date.available2024-10-29T17:50:28Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractThe origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques. Dot-blot hybridization was used to find a genomic affinity between O. viciifolia and 16 diploid Onobrychis species. The hypothesis on the origin of the O. viciifolia was tested using GISH. Dot-blot analyses suggested a genomic affinity between O. viciifolia and four diploid Onobrychis species (O. kachetica, O. supina, O. pallasii, and O. vaginalis). Hybridization signals were observed on O. viciifolia chromosomes when gDNA of O. kachetica, O. supina, O. pallasi, and O. hypargyrea were used as probes. However, the observed chromosomal distribution of hybridization signals did not resemble GISH results. The observed signals colocalized with 35S rDNA or dispearse signals on all chromosomes were observed depending on the probe. Further investigations using more comprehensive and comparative analysis with both coding and repetitive DNA regions may provide a better understanding of the genome composition and evolution of O. viciifolia.
dc.identifier.doi10.28979/jarnas.1428996
dc.identifier.endpage434
dc.identifier.issn2757-5195
dc.identifier.issue2en_US
dc.identifier.startpage426
dc.identifier.trdizinid1247309
dc.identifier.urihttps://doi.org/10.28979/jarnas.1428996
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1247309
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13012
dc.identifier.volume10
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofJournal of advanced research in natural and applied sciences (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOnobrychis
dc.subjectFISH
dc.subjectDot-blot hybridization
dc.subjectGISH
dc.titleFirst Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu