Removal of Pseudomonas fluorescens biofilms from pilot-scale food processing equipment using ozone-assisted cleaning-in-place

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dc.contributor.authorSivri, Goksel Tirpanci
dc.contributor.authorAbdelhamid, Ahmed G.
dc.contributor.authorKasler, David R.
dc.contributor.authorYousef, Ahmed E.
dc.date.accessioned2024-10-29T17:59:17Z
dc.date.available2024-10-29T17:59:17Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractBiofilm formation in food processing environment and within equipment increases the risk of product spoilage and contamination with pathogens. Cleaning-in-place (CIP) operations are useful in removing soils and in sanitizing processing equipment, including eliminating biofilms. However, CIP is a resource-intensive process, particularly in the usage of chemical detergents, heat, and sanitizers. The current study was initiated to investigate the feasibility of integrating ozone into CIP operations to facilitate the elimination of Pseudomonas biofilm, with the long-term goal of decreasing the dependance on conventional cleaning and sanitizing reagents. To investigate integrating ozone into CIP, a robust biofilm of Pseudomonas fluorescens was developed on a pilot-scale food processing equipment after 2 days of incubation in 10% skim milk (skim milk-water mixture, 1:9 v/v) under stagnant conditions, followed by additional 5 days of circulation while feeding 10% fresh skim milk. CIP was applied using water prerinse at 22-25 degrees C, alkaline cleaning with 0.2% potassium hydroxide at 50 degrees C, and a final water rinse. These CIP operations reduced planktonic cell populations below the detection method's limit but did not fully remove P. fluorescens biofilm from either smooth or rough surfaces of the processing equipment. When the CIP process was followed by application of an aqueous ozone step (10 ppm for 10 min), the treatment reduced biofilm cell population, on smooth and rough surfaces, below the recovery method's detection limit (0.9 and 1.4 log CFU/ 100 cm(2), respectively). These findings demonstrate the utility of ozone-assisted CIP in eliminating microbial biofilms on processing equipment, but further research is needed to optimize the use of cleaning agents and the application of ozone.
dc.identifier.doi10.3389/fmicb.2023.1141907
dc.identifier.issn1664-302X
dc.identifier.pmid37125185
dc.identifier.scopus2-s2.0-85159808834
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3389/fmicb.2023.1141907
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14691
dc.identifier.volume14
dc.identifier.wosWOS:000975708700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers in Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbiofilm
dc.subjectPseudomonas spp
dc.subjectcleaning-in-place
dc.subjectozone
dc.subjectdairy industry
dc.subjectfood spoilage
dc.subjectsanitizers
dc.titleRemoval of Pseudomonas fluorescens biofilms from pilot-scale food processing equipment using ozone-assisted cleaning-in-place
dc.typeArticle

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