Bazı kılçıksız brom (Bromus inermis L.) aksesyonlarının çekirdek DNA içeriklerinin belirlenmesi
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Date
2016
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Namık Kemal Üniversitesi
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Abstract
Bu araştırmanın amacı, bazı kılçıksız brom (Bromus inermis L.) aksesyonlarının, flow sitometri yöntemi kullanılarak çekirdek DNA içerikleri, ploidi seviyesi ve kromozom sayılarını belirlemektir. Elde edilen sonuçlara göre; çekirdek DNA içerikleri arasında istatistiksel olarak önemli (P<0.01) farkların olduğu belirlenmiştir. Araştırmada kullanılan kılçıksız brom aksesyonlarının ortalama çekirdek DNA içeriği 11.43 pg/2C ile 26.62 pg/2C arasında değişmektedir. En yüksek çekirdek DNA içeriği 26.62 ve 26.43 pg ile PI 655131 ve PI 628278 nolu aksesyonlarda belirlenirken, en düşük çekirdek DNA içeriği ise aynı önemlilik gurubunda yer alan PI 598583, PI 636580 ve PI 632560 nolu aksesyonlarda sırasıyla 11.43, 11.44 ve 11.45 pg olmuştur. Sonuç olarak, 48 kılçıksız brom aksesyondan 33 aksesyonun tetraploid, 10 aksesyonun octaploid ve 5 aksesyonun ise decaploid olduğu tespit edilmiştir.
The objective of this study was to determinate of nuclear DNA content using flow cytometry, and ploidi levels and chromosome number of some smooth bromegrass. According to the obtained results; it was statistically significant differences (P <0.01) between nuclear DNA contents of smooth bromegrass accessions. Average nuclear DNA contents of smooth bromegrass accessions used in the study varied between 11.43 pg/2C with 26.62 pg/2C. Highest nuclear DNA content was detected as 26.62 pg/2C and 26.43 pg/2C in accessions numbered PI 655131 and PI 628278, respectvely. Lowest nuclear DNA content was detected as 11.43, 11.44 and 11.45 pg/2C in accessions numbered PI 598583, PI 636580 and PI 632560, respectvely. Consequently, 48 smooth bromegrass accessions were identified as 33 tetraploid, 10 octaploid and 5 decaploid smooth bromegrass.
The objective of this study was to determinate of nuclear DNA content using flow cytometry, and ploidi levels and chromosome number of some smooth bromegrass. According to the obtained results; it was statistically significant differences (P <0.01) between nuclear DNA contents of smooth bromegrass accessions. Average nuclear DNA contents of smooth bromegrass accessions used in the study varied between 11.43 pg/2C with 26.62 pg/2C. Highest nuclear DNA content was detected as 26.62 pg/2C and 26.43 pg/2C in accessions numbered PI 655131 and PI 628278, respectvely. Lowest nuclear DNA content was detected as 11.43, 11.44 and 11.45 pg/2C in accessions numbered PI 598583, PI 636580 and PI 632560, respectvely. Consequently, 48 smooth bromegrass accessions were identified as 33 tetraploid, 10 octaploid and 5 decaploid smooth bromegrass.
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Kılçıksız brom, Bromus inermis L., çekirdek DNA içeriği, flow sitometri, kromozom sayısı, Bromegrass, nuclear DNA content, flow cytometry, chromosome number
