Effect of partial replacement of dimethyl sulfoxide with dextran, polyvinylpyrrolidone or polyethylene glycol on post-thaw drone semen quality and queen fertility

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dc.authoridONDER, Nail Tekin/0000-0001-5141-0008
dc.authoridToker, M.Berk/0000-0003-4033-9749
dc.authoridGOKCE, ELIF/0000-0002-7678-3289
dc.authoridCakmak, Ibrahim/0000-0002-8000-5770
dc.authoridNUR, ZEKARIYA/0000-0002-1438-221X
dc.contributor.authorNur, Z.
dc.contributor.authorCakmak, S. S.
dc.contributor.authorCakmak, I.
dc.contributor.authorOnder, N. T.
dc.contributor.authorGokce, E.
dc.contributor.authorUstuner, B.
dc.contributor.authorAlcay, S.
dc.date.accessioned2024-10-29T17:58:51Z
dc.date.available2024-10-29T17:58:51Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractThe aim of this study was to elucidate the toxicity effect of widely used penetrating cryoprotective agent dimethyl sulfoxide (DMSO) on frozen drone semen and fertility. DMSO was replaced totally or partially with either dextran, polyvinylpyrrolidone (PVP), or polyethylene glycol (PEG), which are non-penetrating cryoprotectants. The freezing extender with 12%, 8%, 4% and 0% DMSO were supplemented with 0%, 4%, 8% and 12% of dextran, PVP, or PEG, respectively. For all treatment groups, the final cryoprotectant concentration was 12%. Dextran4, PVP4, and DMSO12 groups with good post-thaw motility, plasma membrane functional integrity that evaluated throughout the study and fresh semen were used for queen instrumental insemination (n=46). Two queen bees (4.4%) died due to manipulation. Alive queens [24 (55%) of 44] had been observed to survive after a week, and 17 (71%) of these queens continued to survive after three months. At the end of three months, 15 (88%) of the alive queens continued to produce worker bees. There were no differences among insemination groups (P>0.05) for the rate of survival for three months and presence of worker broods. After overwintering, there were two queens from the PVP4 and three queens from the Dextran4 group that survived and one queen from PVP4 and two queens from Dextran4 group that continued to produce worker bees. In conclusion, addition of 4% Dextran or PVP as an extender containing 8% DMSO solution improves queen bees' worker egg-laying period, but the same results were not observed for the PEG containing groups.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG215O586]
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) , Project Number: TOVAG215O586.
dc.identifier.doi10.12681/jhvms.34510
dc.identifier.issn1792-2720
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85200395935
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.12681/jhvms.34510
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14530
dc.identifier.volume75
dc.identifier.wosWOS:001282354400017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.ispartofJournal of The Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApis mellifera
dc.subjectcryoprotectants
dc.subjecthoney bee
dc.subjectsemen freezing
dc.subjectqueen fertility
dc.titleEffect of partial replacement of dimethyl sulfoxide with dextran, polyvinylpyrrolidone or polyethylene glycol on post-thaw drone semen quality and queen fertility
dc.typeArticle

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