Key Role for Activin B in Cellular Transformation after Loss of the von Hippel-Lindau Tumor Suppressor

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Küçük Resim

Tarih

2009

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Amer Soc Microbiology

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

The von Hippel-Lindau tumor suppressor gene (VHL) is mutated in clear cell renal cell carcinomas (RCC), leading to the activation of hypoxia-inducible factor (HIF)-mediated gene transcription. Several VHL/HIF targets, such as glycolysis, angiogenesis, cell growth, and chemotaxis of tumor cells, have been implicated in the transformed phenotype of RCC-regulating properties. Here, we show that VHL suppresses key features of cell transformation through downregulation of the HIF-dependent expression of activin B, a member of the transforming growth factor beta superfamily. Activin B expression is repressed by restoration of VHL in VHL-deficient RCC cells and upregulated by hypoxia. RCC tumor samples show increased expression of activin B compared to that in the normal kidney. VHL increases cell adhesion to the extracellular matrix, promotes cell flattening, and reduces invasiveness. These effects are completely phenocopied by RNA interference-mediated knockdown of activin B and reverted by treatment with recombinant activin B. Finally, knockdown of activin B reduces tumor growth of RCC cells in nude mice. Our data indicate that activin B is a key mediator of VHL/HIF-induced transformation in RCC.

Açıklama

Anahtar Kelimeler

Renal-Carcinoma Cells, E-Cadherin, Gene-Product, Vhl, Invasion, Cancer, Growth, Differentiation, Morphogenesis, Metastasis

Kaynak

Molecular and Cellular Biology

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

29

Sayı

7

Künye