The Diagnostic Utility of Flow Cytometry in Celiac Disease Presented Isolated Iron Deficiency Anemia

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dc.contributor.authorTurgut, Burhan
dc.contributor.authorMete, Rafet
dc.contributor.authorOran, Mustafa
dc.contributor.authorAvcı, Burcu Altındağ
dc.date.accessioned2023-04-20T08:08:33Z
dc.date.available2023-04-20T08:08:33Z
dc.date.issued2021
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, İç Hastalıkları Ana Bilim Dalı
dc.description.abstractBackground: Flow cytometric analysis of intestinal intraepithelial lymphocytes contributes to the diagnosis of celiac disease. Celiac disease may present with iron deficiency anemia alone which is considered as one of the forms of atypical celiac disease. In this study, we have aimed to investigate the diagnostic utility of flow cytometric analysis of intraepithelial lymphocytes in this atypical form. Methods: Three groups were formed: the patients with unexplained iron deficiency (group 1), the patients with celiac disease (group 2), and the patients who underwent gastroduodenoscopy for other reasons (group 0). Duodenal biopsy samples were used for flow cytometric analysis of intraepithelial lymphocytes. T cell receptor gammadelta intraepithelial lymphocytes and CD3?/CD103+ intraepithelial lymphocytes were determined with relevant monoclonal antibodies. Sensitivity–specificity calculation was performed to evaluate the usability of flow cytometric variables as diagnostic tests. Results: Group 1 had 22 patients, group 2 had 14 patients, and group 0 had 56 patients. In the comparison of the 3 groups, CD3+/ TCR??+ intraepithelial lymphocytes were found to be higher in celiac patients than other cases. CD3+/TCR??+ intraepithelial lymphocyte was evaluated for its usability as a diagnostic test. The cut-off value of CD3+/TCR??+ intraepithelial lymphocyte as 16.39% according to receiver operating characteristics curve analysis determined celiac disease in 14 of 22 patients in group 1 with 91.7% sensitivity and 80.4% specificity. Conclusions: Although celiac disease is diagnosed with serologic tests and histologic examination, successively, the increase in intestinal CD3+/TCR??+ intraepithelial lymphocytes may be used as a diagnostic test, and it may assist in revealing atypical forms of celiac disease.
dc.identifier.endpage936
dc.identifier.issn2148-5607
dc.identifier.issue11en_US
dc.identifier.startpage932
dc.identifier.trdizinid508512
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/508512
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11345
dc.identifier.volume32
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorMete, Rafet
dc.institutionauthorOran, Mustafa
dc.institutionauthorAvcı, Burcu Altındağ
dc.language.isoen
dc.relation.ispartofTurkish Journal of Gastroenterology
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe Diagnostic Utility of Flow Cytometry in Celiac Disease Presented Isolated Iron Deficiency Anemia
dc.typeArticle

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