A sensitive and disposable electrochemical immunosensor for detection of SOX2, a biomarker of cancer

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dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:10Z
dc.date.available2022-05-11T14:48:10Z
dc.date.issued2017
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel, sensitive, disposable indium tin oxide (ITO)-based electrochemical immunosensor was developed firstly for simple, rapid determination of Sex-determining region Y-box 2 (SOX2). SOX2 is a cancer biomarker and used for detecting small cell lung cancer, lung adenocarcinoma, squamous cell carcinoma, skin cancer, prostate cancer, and breast cancer. In this study, a disposable ITO thin film based electrode was used as working electrode for biosensing the interaction between SOX2 antigen and anti-SOX2 antibody. In this study, carboxyethylsilanetriol (CTES) was also utilized for electrode modifying so as to obtain self-assembled monolayers. The formed self-assembled monolayers were activated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) chemistry and they were used as a heterobifunctional crosslinker and activator, respectively. Anti-SOX2 antibody was used as a biorecognition molecule and was covalently immobilized onto the ITO thin film modified with CTES. Immobilization steps were characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The optimum immobilization conditions such as antibody concentration, antibody and antigen incubation times were examined for the best sensitivity of the immunosensor. Under optimal conditions, this immunosensor had a wide linear detection range (25 fg/mL-2 pg/mL) with a detection limit as low as 7 fg/mL SOX2. Furthermore, the developed SOX2 immunosensor had good storage stability (79.36% of initial activity after 9 weeks), repeatability (3.88% of RSD) and reproducibility (4.25% of RSD). Our developed immunosensor has an acceptable performance for detection of SOX2 antigen, exhibits low detection limit, and has selective and reproducible results in immunoreaction analysis.
dc.description.sponsorshipScientific and Technological Research Council of Turkey, TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey, TUBITAK (Grant number: 113 Z 678)
dc.identifier.doi10.1016/j.talanta.2017.05.048
dc.identifier.endpage170
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid28602290
dc.identifier.scopus2-s2.0-85019570939
dc.identifier.scopusqualityQ1
dc.identifier.startpage162
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2017.05.048
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10608
dc.identifier.volume172
dc.identifier.wosWOS:000404503100022
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSOX2
dc.subjectImmunosensor
dc.subjectITO
dc.subjectCancer biomarker
dc.subjectDisposable biosensor
dc.subjectReduced Graphene Oxide
dc.subjectEndothelial Growth-Factor
dc.subjectIto-Based Immunosensor
dc.subjectLabel-Free
dc.subjectEarly-Diagnosis
dc.subjectReceptor
dc.subjectProtein
dc.subjectExpression
dc.subjectFrequency
dc.subjectElectrode
dc.titleA sensitive and disposable electrochemical immunosensor for detection of SOX2, a biomarker of cancer
dc.typeArticle

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