Label-free and reagent-less electrochemical detection of nucleocapsid protein of SARS-CoV-2: an ultrasensitive and disposable biosensor

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dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-04-20T08:02:25Z
dc.date.available2023-04-20T08:02:25Z
dc.date.issued2022
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel selective and ultrasensitive sandwich biosensor was developed for the detection of nucleocapsid biomarkers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The biosensor fabrication strategy was composed of a combination of gold nanoparticles (AuNPs) electrodeposition and an aldehyde substituted thiophene monomer (3-Thi-Ald) electropolymerization. In this study, an effective surface design was achieved during biosensor fabrication by covalent immobilization of biorecognition elements. The Pthi-Ald polymer not only formed a surface immobilization matrix, but also supported the electrode surface conductivity with its conductive property. The immobilized antibodies on Pthi-Ald polymer could selectively capture nucleocapsid antigen via the specific immunoreaction that resulted in a blockage of the electron transfer through the working electrode because of the resistance of nucleocapsid protein. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and scanning electron microscopy (SEM) techniques were utilized to confirm the step-by-step fabrication procedure of the immunosensor. The developed immunosensor showed good selectivity, high storage stability, acceptable repeatability, and a good reproducibility for nucleocapsid detection. The AuNPs-Pthi-Ald polymer modified biosensor illustrated a good linear dynamic detection range between 0.0015 pg mL(-1) and 150 pg mL(-1) nucleocapsid concentration with a low detection limit of 0.48 fg mL(-1). Moreover, the immunosensor had excellent selectivity, stability, and performance in the analysis of artificial nasal secretion samples. This strategy provided a new aspect for the early screening of SARS-CoV-2 infection.
dc.description.sponsorshipScientific and Technological Research Council of Canakkale Onsekiz Mart University [FIA-2020-3304]
dc.description.sponsorshipThe authors are greatful to the financial support from The Scientific and Technological Research Council of Canakkale Onsekiz Mart University, (FIA-2020-3304).
dc.identifier.doi10.1039/d2nj00046f
dc.identifier.endpage9183
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue19en_US
dc.identifier.scopus2-s2.0-85129912795
dc.identifier.scopusqualityQ2
dc.identifier.startpage9172
dc.identifier.urihttps://doi.org/10.1039/d2nj00046f
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10913
dc.identifier.volume46
dc.identifier.wosWOS:000786695800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofNew Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGold Nanoparticle
dc.subjectCarbon Nanotubes
dc.subjectPolymer
dc.subjectOxide
dc.subjectImmobilization
dc.subjectComposite
dc.subjectAcid
dc.titleLabel-free and reagent-less electrochemical detection of nucleocapsid protein of SARS-CoV-2: an ultrasensitive and disposable biosensor
dc.typeArticle

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