Immobilization of Olive Leaf Extract with Chitosan Nanoparticles as an Adjunct to Enhance Cytotoxicity

Basit Öğe Kaydı
dc.contributor.authorOzdamar, Burcu
dc.contributor.authorSurmeli, Yusuf
dc.contributor.authorSanli-Mohamed, Gulsah
dc.date.accessioned2024-10-29T17:58:31Z
dc.date.available2024-10-29T17:58:31Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesien_US
dc.description.abstractWe immobilized the olive leaf extract (OLE) with chitosannanoparticles(CNPs) by optimizing the effect of various immobilization conditions,and OLE-loaded CNPs (OLE-CNPs) were then elaborately characterizedphysicochemically by scanning electron microscopy (SEM), Fourier transforminfrared (FT-IR) spectroscopy, dynamic light scattering (DLS), andatomic force microscopy (AFM). Under optimal conditions, CNPs wereable to accommodate the OLE with a loading capacity of 97.5%. Theresulting OLE-CNPs had a spherical morphology, and their average diameterwas approximately 100 nm. The cytotoxic influence, cell cycle distribution,and apoptosis stage of OLE and OLE-CNPs were analyzed on lung carcinoma(A549) and breast adenocarcinoma (MCF-7) cell lines. In an in vitrocytotoxic assay, IC50 values of OLE-CNPs were determinedto be 540 & mu;g/mL for A549 and 810 & mu;g/mL for MCF-7. Thetreatment of both A549 and MCF-7 with OLE-CNPs caused the highestcell arrest in G0/G1 in a dose-independent manner. OLE-CNPs affectedcell cycle distribution in a manner different from free OLE treatmentin both cancer cells. A549 and MCF-7 cells were predominantly foundin the late apoptosis and necrosis phases, respectively, upon treatmentof 1000 & mu;M OLE-CNPs. Our results suggest that CNPs enhance theutility of OLEs as nutraceuticals in cancer and that OLE-CNPs canbe utilized as an adjunct to cancer therapy.en_US
dc.identifier.doi10.1021/acsomega.3c01494
dc.identifier.endpage29002en_US
dc.identifier.issn2470-1343
dc.identifier.issue32en_US
dc.identifier.pmid37599944en_US
dc.identifier.scopus2-s2.0-85167876982en_US
dc.identifier.startpage28994en_US
dc.identifier.urihttps://doi.org/10.1021/acsomega.3c01494
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14369
dc.identifier.volume8en_US
dc.identifier.wosWOS:001039807300001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Omegaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGold Nanoparticlesen_US
dc.subjectGreen Synthesisen_US
dc.subjectAnticanceren_US
dc.subjectAntioxidantsen_US
dc.subjectApoptosisen_US
dc.subjectLeavesen_US
dc.subjectLungen_US
dc.subjectPhen_US
dc.titleImmobilization of Olive Leaf Extract with Chitosan Nanoparticles as an Adjunct to Enhance Cytotoxicityen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu