An innovative method for the detection of alpha synuclein, a potential biomarker of Parkinson's disease: quartz tuning fork-based mass sensitive immunosensor design

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dc.authoridARI, Fikret/0000-0002-6104-4467
dc.authoridUNAL, Mehmet Altay/0000-0001-8607-5043
dc.authoridOZKAN, Sibel/0000-0001-7494-3077
dc.contributor.authorKaraboga, Muenteha Nur Sonuc
dc.contributor.authorUnal, Mehmet Altay
dc.contributor.authorAri, Fikret
dc.contributor.authorSezginturk, Mustafa Kemal
dc.contributor.authorOzkan, Sibel Aysil
dc.date.accessioned2024-10-29T17:58:33Z
dc.date.available2024-10-29T17:58:33Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractAn innovative biosensing fabrication strategy has been demonstrated for the first time using a quartz tuning fork (QTF) to develop a practical immunosensor for sensitive, selective and practical analysis of alpha synuclein protein (SYN alpha), a potential biomarker of Parkinson's disease. Functionalization of gold-coated QTFs was carried out in 2 steps by forming a self-assembled monolayer with 4-aminothiophenol (4-ATP) and conjugation of gold nanoparticles (AuNPs). The selective determination range for SYN alpha of the developed biosensor system is 1-500 ng mL-1 in accordance with the resonance frequency shifts associated with a limit of detection of 0.098 ng mL-1. The changes in surface morphology and elemental composition were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDX). The remarkable point of the study is that this QTF based mass sensitive biosensor system can capture the SYN alpha target protein in cerebrospinal fluid (CSF) samples with recoveries ranging from 92% to 104%. An innovative biosensing fabrication strategy has been demonstrated for the first time using a quartz tuning fork to develop a practical immunosensor for sensitive, selective and practical analysis of alpha synuclein protein.
dc.identifier.doi10.1039/d3cp04527g
dc.identifier.endpage5114
dc.identifier.issn1463-9076
dc.identifier.issn1463-9084
dc.identifier.issue6en_US
dc.identifier.pmid38259152
dc.identifier.scopus2-s2.0-85183606244
dc.identifier.scopusqualityQ1
dc.identifier.startpage5106
dc.identifier.urihttps://doi.org/10.1039/d3cp04527g
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14381
dc.identifier.volume26
dc.identifier.wosWOS:001146840900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofPhysical Chemistry Chemical Physics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleAn innovative method for the detection of alpha synuclein, a potential biomarker of Parkinson's disease: quartz tuning fork-based mass sensitive immunosensor design
dc.typeArticle

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