The Role and Antagonistic Effects of miR-16-5p in the Regulation of ADP-Ribosylation Factor-Like Tumor Suppressor Gene 1 in Lung Cancer Cells

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dc.contributor.authorYüksel, Tuğba Nurcan
dc.contributor.authorBozgeyik, Esra
dc.contributor.authorBozgeyik, İbrahim
dc.date.accessioned2024-10-29T17:55:11Z
dc.date.available2024-10-29T17:55:11Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractObjective: ADP-ribosylation factor-like tumor suppressor gene 1 is a member of the Ras superfamily of small guanosine triphosphatases that are known to be involved in multiple regulatory pathways in the multistage development of human cancers. Also, ADP-ribosylation factor-like tumor suppressor gene 1 expression levels have been reported to be dramatically lower in both cancer cell lines and tumor tissues compared to con- trols. Accordingly, defects in the regulation of the ADP-ribosylation factor-like tumor suppressor gene 1 gene seems have key tumor suppressive effects in the formation and development of human cancers including lung cancer. Moreover, microRNAs regulating the expression of ADP-ribosylation factor-like tumor suppressor gene 1 have not been described previously. Accordingly, the present study aimed to reveal the influence of miR-16-5p on the regulation of ADP-ribosylation factor-like tumor suppressor gene 1 gene. Materials and Methods: A549 lung adenocarcinoma cells were used. For the overexpression and silencing experiments of miR-16-5p synthetic microRNA mimics and inhibitors were used, respectively. Gene expres- sion analyses were achieved with the help of quantitative real-time polymerase chain reaction. Results: MiR-16-5p was identified to be predictive target of ADP-ribosylation factor-like tumor suppressor gene 1 and directly targets the expression of ADP-ribosylation factor-like tumor suppressor gene 1 as revealed by the overexpression and silencing experiments. Specifically, it was found that miR-1 6-5p-overexpressed A549 cells showed a decrease in ADP-ribosylation factor-like tumor suppressor gene 1 gene expression, whereas miR- 16-5p-suppressed cells showed an increase in expression. These findings possibly suggest that miR-16-5p is the direct regulatory microRNA that posttranscriptionally regulates the expression of ADP-ribosylation factor-like tumor suppressor gene 1. Conclusion: Collectively, miR-16-5p seems to be a key regulatory molecule involved in the posttranscrip- tional regulation of the ADP-ribosylation factor-like tumor suppressor gene 1, and it might be responsible for the downregulation of this gene in lung cancer.
dc.identifier.doi10.5152/eurasianjmed.2023.23073
dc.identifier.endpage207
dc.identifier.issn1308-8734
dc.identifier.issn1308-8742
dc.identifier.issue3en_US
dc.identifier.startpage204
dc.identifier.trdizinid1266726
dc.identifier.urihttps://doi.org/10.5152/eurasianjmed.2023.23073
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1266726
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13870
dc.identifier.volume55
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofEurasian Journal of Medicine
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLung cancer
dc.subjectcancer
dc.subjectARLTS1
dc.subjectARL11
dc.subjectmiR-16-5p
dc.titleThe Role and Antagonistic Effects of miR-16-5p in the Regulation of ADP-Ribosylation Factor-Like Tumor Suppressor Gene 1 in Lung Cancer Cells
dc.typeArticle

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