Investigation of the Effects of Piperlongumine and Doxorubicin Combined Treatment on Cell Death via PTEN in HeLa Cells

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dc.contributor.authorGüzelel, Gonca
dc.contributor.authorAkalan, Hande
dc.contributor.authorBilgen, Türker
dc.contributor.authorŞirin, Duygu Yaşar
dc.date.accessioned2024-10-29T17:55:13Z
dc.date.available2024-10-29T17:55:13Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractDoxorubicin (Dox), which is used in treating many types of cancer including cervix cancer nevertheless, its effect alone is low especially in recurrent cases. Therefore, investigating of agents that can increase the impact of Dox continues. The aim of the present study is to answer the question: Can Piperlongumine (PL) a natural alkaloid cause an increase in the efficacy of Dox in the HeLa cell line? In this study, the effects of Dox and PL on cell viability by MTT and Acridine orange/propidium iodide staining, and expression levels of the PTEN (Phosphatase and tensin homolog 10) gene by Real-Time PCR and Western-Blot were evaluated in HeLa cells. It was determined that PL combined with Dox increased cell death and suppressed cell proliferation. The PTEN gene expression was decreased in all experimental groups, but the PTEN protein phosphorylation increased in cultures treated with PL and when Dox/PL was combined. The fact that PL application increases the activation of PTEN, which is a tumor suppressor. This indicates that it can be used to increase the effectiveness of Dox in the treatment.
dc.identifier.doi10.53433/yyufbed.1258515
dc.identifier.endpage11
dc.identifier.issn1300-5413
dc.identifier.issn2667-467X
dc.identifier.issue1en_US
dc.identifier.startpage1
dc.identifier.trdizinid1233233
dc.identifier.urihttps://doi.org/10.53433/yyufbed.1258515
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1233233
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13895
dc.identifier.volume29
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofYüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDoxorubicin
dc.subjectPTEN
dc.subjectPiperlongumine
dc.titleInvestigation of the Effects of Piperlongumine and Doxorubicin Combined Treatment on Cell Death via PTEN in HeLa Cells
dc.typeArticle

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