The effect of q-RT-PCR analysis method on saline gargle samples in SARS-CoV-2 clinical diagnostic methods

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dc.contributor.authorKömürcü, Selen Zeliha Mart
dc.contributor.authorArtık, Yakup
dc.contributor.authorUyar, Yavuz
dc.contributor.authorHizel, Nedret
dc.contributor.authorSur, Haydar
dc.contributor.authorKocadag, Zeynal Abidin
dc.contributor.authorKazezoğlu, Cemal
dc.date.accessioned2023-04-20T08:04:13Z
dc.date.available2023-04-20T08:04:13Z
dc.date.issued2022
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractCOVID-19 is a devastating disease, and its control is difficult due to its high transmissibility rate and a long incubation average period (6.4 days). Additionally, more than half of the infected patients were asymptomatic young people or children. The asymptomatic virus transmission is the actual challenge to controlling the disease. Because of limited treatment options, diagnosis techniques have been the first focus all over the world, involving q-RT-PCR as a gold standard, serological tests, point of care studies, or RT-LAMP. Generally, nasopharyngeal, and oropharyngeal samples are preferred clinically as sources. However, alternative sources are being researched, particularly for healthcare professionals who have difficulty taking samples, patients who are afraid of giving samples, and pediatric patients. Herein, physiological saline has been utilized to offer an alternative source besides the swab samples for use in q-RT-PCR. In this study, 212 randomly chosen patients' samples were studied, and we evaluated the concordance and accurate q-RT-PCR results in two different sources, obtained from swab and gargle samples of patients. Herein, physiological saline is utilized, which is widely used medically as a recommended irrigating and wound dressing solution. We obtained in our experiments with this method, the confidence interval determines 74.50% positivity when compared to the routine q-RT-PCR procedure as summarized. In addition, when only the gargle sampling method is studied in low-income countries, the cost of testing for COVID-19 will decrease significantly. Because this method does not require vNAT or VTM transport solution sterile swab sticks as shown. The plastic container with a lid in which the patient can gargle with SF and spit it out is an ideal method for this. Additionally, it provides a great cost-benefit in low-income countries.
dc.description.sponsorshipPrivate Viromed Istanbul Central Laboratory; Imaging Center, Istanbul, Turkey
dc.description.sponsorshipThis study was supported by the Private Viromed Istanbul Central Laboratory and Imaging Center, Istanbul, Turkey.
dc.identifier.doi10.29333/ejgm/12511
dc.identifier.issn2516-3507
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85140431999
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.29333/ejgm/12511
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11031
dc.identifier.volume19
dc.identifier.wosWOS:000867861500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorUyar, Yavuz
dc.language.isoen
dc.publisherModestum Ltd
dc.relation.ispartofElectronic Journal of General Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSars-Cov-2
dc.subjectQ-Rt-Pcr
dc.subjectSaline
dc.subjectPhysiologic Serum
dc.subjectGargle
dc.titleThe effect of q-RT-PCR analysis method on saline gargle samples in SARS-CoV-2 clinical diagnostic methods
dc.typeArticle

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