A nano-composite based regenerative neuro biosensor sensitive to Parkinsonism-associated protein DJ-1/Park7 in cerebrospinal fluid and saliva
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, we developed an electrochemical-based single-use neurobiosensor based on multiwalled carbon nanotube (MWCNT)-gold nanoparticle (AuNP) nanocomposite doped, 11-amino-1-undecanethiol (11-AUT)-modified polyethylene terephthalate coated indium tin oxide (ITO-PET) electrodes. This electrode was used for the sensitive determination of DJ-1, a protein responsible for mitochondrial dysfunction in Parkinson's disease (PD) with the task of eliminating oxidative stress. The design strategy and analytical studies for the neurobiosensor were monitored with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and single frequency impedance (SFI) techniques. The selective determination range for DJ-1 of the developed neurobiosensor system is 4.7–4700 fg mL?1 in accordance with the charge transfer resistance (Rct) associated with a limit of detection of 0.5 fg mL?1. Since changes in the expression of DJ-1 protein is particularly important in cerebrospinal fluid (CSF) and saliva, the ability of the developed neurobiosensor system to detect the DJ-1 protein in these media was tested by the standard addition method. The statistical results show that the biosensor decorated with MWCNT-AuNP-AUT may be recommended for the selective determination of DJ-1 protein. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Biosensor, DJ-1, MWCNT, Neurodegeneration, Parkinson's disease, Biosensors, Brain, Charge transfer, Cyclic voltammetry, Electrochemical electrodes, Electrochemical impedance spectroscopy, Gold nanoparticles, Multiwalled carbon nanotubes (MWCN), Nanocomposites, Neurodegenerative diseases, Plastic bottles, Proteins, Tin oxides, Analytical studies, Cerebro spinal fluids, Charge transfer resistance, Limit of detection, Mitochondrial dysfunction, Parkinson's disease, Selective determination, Standard addition method, Cerebrospinal fluid, gold nanoparticle, indium tin oxide, multi walled nanotube, nanocomposite, polyethylene terephthalate, protein deglycase DJ-1, carbon nanotube, gold, metal nanoparticle, nanocomposite, protein deglycase DJ-1, Article, cerebrospinal fluid, controlled study, cyclic voltammetry, disorders of mitochondrial functions, impedance spectroscopy, oxidative stress, Parkinson disease, protein expression, saliva, cerebrospinal fluid, chemistry, electrochemistry, genetic procedures, human, metabolism, Parkinson disease, procedures, Biosensing Techniques, Electrochemistry, Gold, Humans, Metal Nanoparticles, Nanocomposites, Nanotubes, Carbon, Parkinson Disease, Protein Deglycase DJ-1, Saliva
Kaynak
Bioelectrochemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
138