A highly selective electrochemical immunosensor based on conductive carbon black and star PGMA polymer composite material for IL-8 biomarker detection in human serum and saliva

Basit Öğe Kaydı
dc.authorscopusid55571487900
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, MUHAMMET/ABA-9027-2020
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Muhammet
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:13Z
dc.date.available2022-05-11T14:48:13Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA new approach to enhance the electrochemical performance of biosensor was attempted by using Super P (c) carbon black/Star polymer composite material. In this study, we developed an electrochemical IL 8 biosensor by modification with a conductive composite including Super P, polyvinylidene fluoride (PVDF) and star polymer (SPGMA) of disposable ITO electrode surface. The Super P carbon black as carbonaceous material had a high conductivity and was used for the enhancement of electron transfer between electrode surface and electrolyte. Anti-IL 8 antibodies were utilized as biorecognition molecules and bound to epoxy groups of star polymer covalently. The chemical characterization of antibody immobilization on this composite was performed by using Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy. The characterizations of stepwise modification of this immunosensor were performed by electrochemical techniques such as Electrochemical Impedance Spectroscopy (EIS), Cyclic Voltammetry (CV) and Single Frequency Impedance (SFI); and morphological techniques such as Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Several variables that affect the immunosensor performance were optimized. Under optimum conditions, a wide linear range 0.01-3 pg/mL and low detection limit 3.3 fg/mL were obtained. Super P-star polymer composite modified immunosensor was easy, sensitive, cheap and reliable analytical method for IL 8 detection. The applicability of the proposed immunosensor to determine IL 8 in saliva and serum samples were examined. The results of biosensor and Enzyme-linked Immunosorbent Assay (ELISA) kit were in compatible. Consequently, it was concluded that the electrochemical immunosensor offers a potential approach for IL 8 detection in clinical applications.
dc.description.sponsorshipScientific and Technological Research Council of by Namik Kemal University, NKU-BAP [NKUBAP.00.DS.17.114]
dc.description.sponsorshipThe financial support from Scientific and Technological Research Council of by Namik Kemal University, NKU-BAP (Grant number: NKUBAP.00.DS.17.114) is gratefully acknowledged.
dc.identifier.doi10.1016/j.bios.2018.07.010
dc.identifier.endpage728
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid30014946
dc.identifier.scopus2-s2.0-85050129633
dc.identifier.scopusqualityQ1
dc.identifier.startpage720
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.07.010
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10613
dc.identifier.volume117
dc.identifier.wosWOS:000442191900089
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProtein IL 8
dc.subjectElectrochemical impedance spectroscopy
dc.subjectStar-polymer
dc.subjectPoly(glycidyl methacrylate)
dc.subjectConductive Super P-circle Carbon Black
dc.subjectSquamous-Cell Carcinoma
dc.subjectLabel-Free
dc.subjectImpedance Spectroscopy
dc.subjectRaman-Spectroscopy
dc.subjectCancer Biomarkers
dc.subjectGraphene Oxide
dc.subjectInterleukin-8
dc.subjectProtein
dc.subjectBiosensor
dc.subjectDiagnosis
dc.titleA highly selective electrochemical immunosensor based on conductive carbon black and star PGMA polymer composite material for IL-8 biomarker detection in human serum and saliva
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
10613-2.pdf
Boyut:
1.6 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu