COMBINING DIMETHYL SULPHOXIDE (DMSO) WITH DIFFERENT CRYOPROTECTANTS ENSURES BETTER CARTILAGE CELL CRYOPRESERVATION

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dc.authorid0000-0002-2789-0635
dc.authorwosidarat, sezen/ABA-7370-2020
dc.contributor.authorTüten Sevim, Emel
dc.contributor.authorArat, Sezen
dc.date.accessioned2022-05-11T14:07:12Z
dc.date.available2022-05-11T14:07:12Z
dc.date.issued2021
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractBACKGROUND: DMSO is a cryoprotective agent (CPA) that is widely used in the cryopreservation of cells. However, evidence suggests that it is more effective when combined with other CPAs. OBJECTIVE: To investigated the effect of combining serum and balanced solutions with DMSO for cryopreservation. MATERIALS AND METHODS: Cartilage cells cultured with Dulbecco's Modified Eagle Medium (DMEM) medium were taken from the ears of adult cattle. Then, these cells were frozen by supplementation with different concentrations of fetal bovine serum (FBS) and different balanced solutions with DMSO. RESULTS: The highest cell viability was obtained using freezing solutions containing 10% DMSO and 40% serum, in dextran 40 or dextrose solution or DMEM. CONCLUSION: Our results indicated that serum concentration is important for cell viability and that supplementing DMSO with dextran or dextrose or DMEM benefits the cryopreservation of bovine cartilage cells.
dc.identifier.endpage226
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue4en_US
dc.identifier.pmid35363841
dc.identifier.startpage220
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5009
dc.identifier.volume42
dc.identifier.wosWOS:000667196600004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorArat, Sezen
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcartilage
dc.subjectcell viability
dc.subjectcryopreservation
dc.subjectDMSO
dc.subjectsugars
dc.subjectMesenchymal Stem-Cells
dc.subject2-Cell Mouse Embryos
dc.subjectHydroxyethyl Starch
dc.subjectConservation Programs
dc.subjectSerum
dc.subjectCulture
dc.subjectTissue
dc.subjectDimethylsulfoxide
dc.subjectKeratinocytes
dc.subjectPreservation
dc.titleCOMBINING DIMETHYL SULPHOXIDE (DMSO) WITH DIFFERENT CRYOPROTECTANTS ENSURES BETTER CARTILAGE CELL CRYOPRESERVATION
dc.typeArticle

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