EErwinia amylovora [(Burr.) Winslow et al.)]'nın neden olduğu ateş yanıklığı hastalığının biyolojik mücadelesinde bakteriyofaj ve yararlı mikroorganizmaların kullanımı
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tekirdağ Namık Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Ateş yanıklığı hastalığı yumuşak çekirdekli meyve ağaçlarının en tahripkâr bakteriyel hastalığıdır. Çalışmada farklı il ve ilçelerden izole edilen patojen klasik ve moleküler yöntemlerle tanılanmış ve biyolojik mücadelesi üzerine çalışılmıştır. Bu kapsamda epifitik bakteri ve maya izolasyonu yapılmış, toplamda 503 adet aday antagonist izolat elde edilmiştir. İzolatların hastalığa karşı etkileri; in vitro, yarı in vivo ve in vivo koşullarda araştırılmıştır. Başarılı 11 adet antagonist bakteri izolatıyla, in vivo koşullarda 2 farklı sera denemesi gerçekleştirilmiş, iki antagonist (AKMrArC-1 ve BİBoEY-3) oldukça başarılı bulunmuş ve iki denemede hastalığı sırasıyla; %91,7-90,0 ve %85,0-88,3 engellemiştir. MALDİ-TOF MS ile tür düzeyinde yapılan tanılama sonucunda bu antagonistler Pseudomonas graminis olarak tanılanmıştır. Bu izolatlar hastalığı baskılamada %80 Fosetyl-Al kimyasalından, Bacillus subtilis QST 713 biyolojik preparatından ve streptomisin sülfat antibiyotiğinden daha etkili olmuştur. Bakteriyofaj izolasyonları için hastalığın görüldüğü bahçelerde sörveyler yapılmış, 59 adet bakteriyofaj izole edilmiştir. Fajların hastalığa karşı etkileri; yarı in vivo ve in vivo koşullarda araştırılmıştır. Sera koşullarında yapılan in vivo deneme sonuçlarına göre, birinci sera denemesinde, bakteriyofaj kokteyllerinin hastalığı engelleme oranları %41,67 ile %68,33; ikinci sera denemesinde, %51,67 ile %68,33 arasında değişiklik göstermiştir. Her iki sera denemesinde de kokteyl faj uygulamaları Bacillus subtilis QST 713 biyolojik preparatı ve streptomisin sülfat antibiyotiğinden daha etkili bulunmuştur. Bu doktora çalışması, ateş yanıklığı hastalığının biyolojik mücadelesinde bakteriyofajların kullanım olanaklarının belirlenmesine yönelik ülkemizdeki ilk çalışmadır.
Fire blight is the most destructive bacterial disease of stone fruit trees. In this study, the pathogen isolated from different provinces and districts was identified by classical and molecular methods and its biological control was studied. In this context, epiphytic bacteria and yeasts were isolated and a total of 503 candidate antagonist isolates were obtained. The effects of the isolates against the disease were investigated under in vitro, semi-in vivo and in vivo conditions. Two different greenhouse trials were conducted with 11 successful antagonist bacterial isolates under in vivo conditions. Two antagonists (AKMrArC-1 and BIBoEY-3) were found to be highly successful and prevented the disease by 91,7-90,0% and 85,0-88,3%, respectively. These antagonists were identified as Pseudomonas graminis by MALDI-TOF MS at species level. These isolates were more effective than 80% Fosetyl-Al chemical, Bacillus subtilis QST 713 biological preparation and streptomycin sulphate antibiotic in suppressing the disease. For bacteriophage isolation, surveys were carried out in the gardens where the disease was observed and 59 bacteriophages were isolated. The effects of phages against the disease were investigated under semi-in vivo and in vivo conditions. According to the results of in vivo experiments conducted under greenhouse conditions, the disease inhibition rates of bacteriophage cocktails varied between 41.67% and 68.33% in the first greenhouse experiment and between 51.67% and 68.33% in the second greenhouse experiment. In both greenhouse experiments, cocktail phage treatments were more effective than Bacillus subtilis QST 713 biological preparation and streptomycin sulphate antibiotic. This PhD study is the first study in our country to determine the possibilities of using bacteriophages in the biological control of fire blight disease.
Fire blight is the most destructive bacterial disease of stone fruit trees. In this study, the pathogen isolated from different provinces and districts was identified by classical and molecular methods and its biological control was studied. In this context, epiphytic bacteria and yeasts were isolated and a total of 503 candidate antagonist isolates were obtained. The effects of the isolates against the disease were investigated under in vitro, semi-in vivo and in vivo conditions. Two different greenhouse trials were conducted with 11 successful antagonist bacterial isolates under in vivo conditions. Two antagonists (AKMrArC-1 and BIBoEY-3) were found to be highly successful and prevented the disease by 91,7-90,0% and 85,0-88,3%, respectively. These antagonists were identified as Pseudomonas graminis by MALDI-TOF MS at species level. These isolates were more effective than 80% Fosetyl-Al chemical, Bacillus subtilis QST 713 biological preparation and streptomycin sulphate antibiotic in suppressing the disease. For bacteriophage isolation, surveys were carried out in the gardens where the disease was observed and 59 bacteriophages were isolated. The effects of phages against the disease were investigated under semi-in vivo and in vivo conditions. According to the results of in vivo experiments conducted under greenhouse conditions, the disease inhibition rates of bacteriophage cocktails varied between 41.67% and 68.33% in the first greenhouse experiment and between 51.67% and 68.33% in the second greenhouse experiment. In both greenhouse experiments, cocktail phage treatments were more effective than Bacillus subtilis QST 713 biological preparation and streptomycin sulphate antibiotic. This PhD study is the first study in our country to determine the possibilities of using bacteriophages in the biological control of fire blight disease.
Açıklama
06.11.2024 tarihine kadar kullanımı yazar tarafından kısıtlanmıştır.
Fen Bilimleri Enstitüsü, Bitki Koruma Ana Bilim Dalı
Fen Bilimleri Enstitüsü, Bitki Koruma Ana Bilim Dalı
Anahtar Kelimeler
Ziraat, Agriculture
