Ultrasensitive detection of NSE employing a novel electrochemical immunosensor based on a conjugated copolymer

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:33Z
dc.date.available2024-10-29T17:58:33Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractIn the current study a simple and highly specific label-free impedimetric neuron specific enolase (NSE) immunosensor based on a copolymer matrix-coated disposable electrode was designed and tested. The copolymer matrix was prepared using a very conductive EDOT monomer and semi-conductive thiophene-bearing epoxy groups (ThEp), and the combination of the two monomers enhanced the conductivity and protein loading capacity of the electrode surface. The P(ThEp-co-EDOT) copolymer matrix was prepared via a drop-casting process and anti-NSE recognition biomolecules were immobilized directly on the epoxy groups of the copolymer. After the coupling of NSE molecules on the P(ThEp-co-EDOT) copolymer matrix-coated electrode surface, the charge transfer resistance (Rct) of the biosensor changed dramatically. These changes in Rct were proportional to the NSE molecule amounts captured by anti-NSE molecules. Under optimized experimental conditions, the increment in the Rct value was proportional to the NSE concentration over a range of 0.01 to 25 pg mL-1 with a detection limit (LOD) of 2.98 x 10-3 pg mL-1. This copolymer-coated electrode provided a lower LOD than the other biosensors. In addition, the suggested electrochemical immuno-platform showed good selectivity, superior reproducibility, long-term stability, and high recovery of NSE in real serum (95.64-102.20%) and saliva (95.28-105.35%) samples. These results showed that the present system had great potential for electrochemical biosensing applications. A P(ThEp-co-EDOT) copolymer matrix was synthesized and utilized as a platform to construct a new NSE biosensor.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namimath;k Kemal University; [NKUBAP.00. DS.18.180]
dc.description.sponsorshipThis work was financially promoted by the Scientific and Technological Research Council (NKUBAP.00. DS.18.180) of Tekirdag Nam & imath;k Kemal University.
dc.identifier.doi10.1039/d3an01602a
dc.identifier.endpage1644
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue5en_US
dc.identifier.pmid38305417
dc.identifier.scopus2-s2.0-85183962997
dc.identifier.scopusqualityQ1
dc.identifier.startpage1632
dc.identifier.urihttps://doi.org/10.1039/d3an01602a
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14380
dc.identifier.volume149
dc.identifier.wosWOS:001155373200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofAnalyst
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNeuron-Specific Enolase
dc.subjectLung-Cancer
dc.subjectTumor-Marker
dc.subjectBiomarkers
dc.subjectBiosensors
dc.subjectDiagnosis
dc.subjectLiquid
dc.titleUltrasensitive detection of NSE employing a novel electrochemical immunosensor based on a conjugated copolymer
dc.typeArticle

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