Detection of Brucella among Voluntary Blood Donors in Turkey by Using a New Real Tune PCR Method

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dc.authorscopusid55485574600
dc.authorscopusid20433626100
dc.authorscopusid15135117000
dc.authorscopusid25226509700
dc.contributor.authorYavuz, M. Tevfik
dc.contributor.authorKaya, Demet
dc.contributor.authorBehçet, Mustafa
dc.contributor.authorAkşit, Hasan
dc.date.accessioned2022-05-11T14:42:10Z
dc.date.available2022-05-11T14:42:10Z
dc.date.issued2012
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractBnicellosis is a worldwide zoonosis also the incidence of which has decreased in developed countries it is still effective in Asia, Africa, Central America and the Mediterranean basin (Turkey inside) that have insufficient public health and domestic animal health programs. There are many reports about Brucella endemics in Turkey. Although, human to human transmission is not frequently, it has been reported in association with blood transfusion, bone marrow transplantation and transplacental transmission in worldwide and Turkey. Human brucellosis is diagnosed based on clinical findings and laboratory studies that include bacteriological and serological tests. The conventional serological tests are insufficiently sensitive and specific to be used individually and often leading to difficulty in interpreting the results. In this study, researchers aimed the detection of possible Brucella carriers without manifest chnHcal finding among voluntary blood donors by using new real time PCR Method. Total 632 voluntary donor blood samples for four month were investigated for Brucella. The samples were subjected to both Wright and ELISA tests for Brucella. In the second step, leucocytes were separated from all samples and performed real time PCR based on BioRad I-Cycler. Although, the region is not high risk epidemiologic area for Brucellosis in. Turkey 4 out of total 632 samples were detected by Wright agglutination and Brucella IgG, IgM ELISA while 2 positive samples were found by I-Cycler based real time PCR detection. All of positive samples were collected from rural area donors. Even it is not in high prevalence, this results and earlier studies were shown brucellosis could be a risk for blood transfusion. Researchers advice especially donors from coming rural area must be performed a questionnaire for brucellosis findings and Brucella screening tests must be performed for blood donors in endemic areas.
dc.identifier.doi10.3923/javaa.2012.3247.3250
dc.identifier.endpage3250
dc.identifier.issn1680-5593
dc.identifier.issue17en_US
dc.identifier.scopus2-s2.0-84873023903
dc.identifier.scopusqualityN/A
dc.identifier.startpage3247
dc.identifier.urihttps://doi.org/10.3923/javaa.2012.3247.3250
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9259
dc.identifier.volume11
dc.identifier.wosWOS:000314149100036
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaya, Demet
dc.language.isoen
dc.publisherMedwell Online
dc.relation.ispartofJournal of Animal and Veterinary Advances
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBrucella
dc.subjectblood transmission
dc.subjectreal time PCR
dc.subjectdonor
dc.subjectagglutination
dc.subjectCongenital Brucellosis
dc.subjectDiagnosis
dc.subjectMelitensis
dc.subjectInfection
dc.subjectAbortus
dc.subjectKuwait
dc.titleDetection of Brucella among Voluntary Blood Donors in Turkey by Using a New Real Tune PCR Method
dc.typeArticle

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