The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD

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dc.contributor.authorBostancieri, Nuray
dc.contributor.authorBakir, Kemal
dc.contributor.authorKul, Seval
dc.contributor.authorEralp, Ayhan
dc.contributor.authorKayalar, Ozgecan
dc.contributor.authorKonyalilar, Nur
dc.contributor.authorRajabi, Hadi
dc.date.accessioned2024-10-29T17:43:36Z
dc.date.available2024-10-29T17:43:36Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesien_US
dc.description.abstractBackground: Although studies suggest a deficiency in stem cell numbers in chronic airway diseases such as chronic obstructive pulmonary disease (COPD), the role of bronchial epithelial progenitor/stem (P/S) cells is not clear. The objectives of this study were to investigate expression of progenitor/stem (P/S) cell markers, cytokeratin (CK) 5, CK14 and p63 in bronchial epithelial explants and cell cultures obtained from smokers with and without COPD following multiple outgrowths, and to study this effect on bronchial epithelial cell (BEC) proliferation. Methods: Bronchial epithelial explants were dissected from lung explants and cultured on coverslips. Confluent cultures were obtained after 3–4 weeks’ (transfer, Tr1), explants were then transferred and cultured for a second (Tr2) and third (Tr3) time, respectively. At each stage, expression of CK5, CK14 and p63 in explants and BEC were determined by immunostaining. In parallel experiments, outgrowing cells from explants were counted after 4wks, and explants subsequently transferred to obtain new cultures for a further 3 times. Results: As the transfer number advanced, CK5, CK14 and p63 expression was decreased in both explants and BEC from both smokers without COPD and patients with COPD, with a more pronounced decrease in BEC numbers in the COPD group. Total cell numbers cultured from explants were decreased with advancing outgrowth number in both groups. Smoking status and lung function parameters were correlated with reduced P/S marker expression and cell numbers. Conclusion: Our findings suggest that the number of P/S cells in airway epithelium may play a role in the pathogenesis of COPD, as well as a role in the proliferation of airway epithelial cells, in vitro. Copyright © 2023 Bostancieri, Bakir, Kul, Eralp, Kayalar, Konyalilar, Rajabi, Yuncu, Yildirim and Bayram.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (111S029)en_US
dc.identifier.doi10.3389/fmed.2023.1118715
dc.identifier.issn2296-858X
dc.identifier.scopus2-s2.0-85175373916en_US
dc.identifier.urihttps://doi.org/10.3389/fmed.2023.1118715
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12505
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFrontiers Media SAen_US
dc.relation.ispartofFrontiers in Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCOPDen_US
dc.subjectcytokeratin 14en_US
dc.subjectcytokeratin 5en_US
dc.subjectp63en_US
dc.subjectprimary bronchial epithelial cell cultureen_US
dc.subjectprogenitor/stem cellen_US
dc.subjectsmokeren_US
dc.titleThe effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPDen_US
dc.typeArticleen_US

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