Determination of calreticulin using Fe3O4@AuNPs core-shell functionalized with PT(COOH)(2) polymer modified electrode: A new platform for the impedimetric biosensing of cancer biomarkers

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-04-20T08:02:25Z
dc.date.available2023-04-20T08:02:25Z
dc.date.issued2022
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractAn innovative biosensing design strategy was utilized for the first time to develop a label-free and practical immunosensor for ultrasensitive analysis of calreticulin (CRT), a potential biomarker of breast carcinoma. Unlike strategies used in literature, a conjugated polymer carrying carboxyl groups on its backbone was synthesized and utilized as an immobilization matrix. This immunosensor design strategy was composed of the attachment of anti-CRT antibodies onto iron oxide nanoparticles@gold nanoparticles@poly(3-thiophenemalonic acid) polymer (Fe3O4@AuNPs@PT(COOH)(2)) functionalized indium tin oxide (ITO) electrode through carbodiimide coupling reaction. EIS measurement results illustrated significant changes in charge transfer resistance (R-ct) on immunoreaction between anti-CRT and CRT proteins. The proposed immunosensor enabled the detection of CRT over the range of 0.02 pg/mL and 100 pg/mL with a correlation coefficient of 0.9994 under optimal conditions. The detection limit (LOD), the quantification limit (LOQ) and the sensitivity were also determined to be 8.2 fg/mL, 27.4 fg/mL and 270 k Omega fg mL(-1)cm(2), respectively. Furthermore, it also exhibited good repeatability, excellent reproducibility, long storage stability and reusability. Experimental results analyzed with T-test to compare the means of the repeatability and reproducibility data, and F-test to the distribution of the repeatability and reproducibility data. According to T and F-tests, the experimental results achieved from the studies with the suggested immunosensor were relatively satisfactory. Furthermore, this immunosensor was tested to measure CRT levels in human serum and spiked human serum samples, and acceptable recovery rates ranging from 94.05% to 106.62% were obtained. In sum, considering its fast and easy-to-fabricate properties, this new strategy offers a potential tool for CRT biomarker detection.
dc.description.sponsorshipScientific and Technological Research Council of Canakkale Onsekiz Mart University [FHD-2021-3533]
dc.description.sponsorshipThis research was also financially supported by The Scientific and Technological Research Council of Canakkale Onsekiz Mart University (FHD-2021-3533).
dc.identifier.doi10.1016/j.snb.2022.132099
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85131444262
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2022.132099
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10912
dc.identifier.volume367
dc.identifier.wosWOS:000833522700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCalreticulin
dc.subjectIron Oxide Nanoparticles
dc.subjectFe3o4@Aunps@Pt(Cooh)(2) Core-Shell
dc.subjectImpedimetric Analysis
dc.subjectElectrochemical Immunosensor
dc.subjectFe3o4 Nanoparticles
dc.subjectSerum
dc.subjectRaman
dc.subjectTransition
dc.titleDetermination of calreticulin using Fe3O4@AuNPs core-shell functionalized with PT(COOH)(2) polymer modified electrode: A new platform for the impedimetric biosensing of cancer biomarkers
dc.typeArticle

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