Selective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly(pyrrole) polymer modified disposable ITO electrode

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:24Z
dc.date.available2022-05-11T14:07:24Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractAn ultrasensitive, specific and label-free immunosensor was developed to determine the lowest alteration of neuron specific enolase antigen (NSE), the standard biomarker of lung cancer patients. This immunosensor was the first biosensor which was fabricated by utilizing epoxy-substituted-polypyrrole P(Pyr-Epx) polymer as an immobilization platform. The fabrication steps of the immunosensor were followed by using electrochemical studies (electrochemical impedance spectroscopy and cyclic voltammetry) and these techniques were applied to characterize the binding interactions. Scanning electron microscopy and atomic force microscopy analyses were performed to investigate the changes formed on the indium tin oxide electrode surface morphology and electrode surface structure. The optimization of the experimental parameters and the analytical performance of the proposed immunosensor were widely evaluated. Under optimum experimental conditions, the change in impedimetric signal was determined to follow the specific biointeraction between anti-NSE antibody and NSE antigen. The sensing ability of the proposed immunosensor for NSE detection showed a wide linear detection range of 0.02–7.5 pg/mL, with a low detection limit of 6.1 fg/mL. This impedimetric immunosensor also demonstrated highly repeatable and reproducible responses, and reliable results in the analysis of human serum samples with recoveries between 98.29 % and 102.81 %. In addition, the designed immunosensor could be used for detection of lung cancer biomarkers after simple dilution of serum samples with phosphate buffer. © 2019 Elsevier B.V.
dc.identifier.doi10.1016/j.snb.2019.127613
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85076839961
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2019.127613
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5096
dc.identifier.volume306
dc.identifier.wosWOS:000507459500038
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpoxy-substituted-polypyrrole P(Pyr-Epx) polymer
dc.subjectImpedimetric immunosensor
dc.subjectNeuron specific enolase
dc.subjectAntigens
dc.subjectAtomic force microscopy
dc.subjectBinding energy
dc.subjectBiological organs
dc.subjectBiomarkers
dc.subjectCyclic voltammetry
dc.subjectDiseases
dc.subjectElectrochemical impedance spectroscopy
dc.subjectImmunosensors
dc.subjectIndium metallography
dc.subjectMorphology
dc.subjectPolymers
dc.subjectPolypyrroles
dc.subjectScanning electron microscopy
dc.subjectStructural optimization
dc.subjectSurface morphology
dc.subjectTin metallography
dc.subjectTin oxides
dc.subjectElectrochemical immunosensing
dc.subjectElectrochemical studies
dc.subjectExperimental parameters
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide electrodes
dc.subjectLinear detection ranges
dc.subjectNeuron specific enolase
dc.subjectSubstituted polypyrrole
dc.subjectElectrochemical electrodes
dc.titleSelective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly(pyrrole) polymer modified disposable ITO electrode
dc.typeArticle

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