A disposable and ultrasensitive ITO based biosensor modified by 6-phosphonohexanoic acid for electrochemical sensing of IL-1 beta in human serum and saliva

Basit Öğe Kaydı
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:12Z
dc.date.available2022-05-11T14:48:12Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, we constructed a new and sensitive ITO based electrochemical immunosensor for detection of interleukin 1 beta(IL-1 beta), a cancer biomarker found in serum and saliva. 6-phosphonohexanoic acid (PHA) was used as a biomolecule immobilization matrix for the first time. Anti-IL-1 beta antibody was utilized as a biorecognition molecule that immobilized onto carboxyl groups of 6-phosphohexanoic acid (PHA) via amide bond. Selective interaction between anti-IL-1 beta antibodies and IL-1 beta antigens was investigated by Electrochemical Impedance Spectroscopy (EIS), Cyclic Voltammetry (CV) and Single Frequency Impedance (SFI) methods. The surface characterization of the immunosensor was performed by fourier transform infrared spectroscopy (FTIR), raman spectroscopy, scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) in order to illustrate individual steps of biosensor construction. Under the optimized experimental conditions, the change in impedance was proportional to IL-1 beta concentrations in the range of 0.025-3 pg/mL (R-2 = 0.99) with detection limit of 7.5 fg/mL. The reproducibility, repeatability, stability, and specificity of the developed immunosensor were analyzed. In addition, the developed immunosensor was successfully utilized for the determination of IL-1 beta in serum and saliva samples by using the standard addition method with recoveries of 96.7-105.4%. This immunosensor was applicable for the requirements of routine analysis with respect to performance, functionality and cost. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2018.07.055
dc.identifier.endpage50
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid30322551
dc.identifier.scopus2-s2.0-85050743993
dc.identifier.scopusqualityQ1
dc.identifier.startpage41
dc.identifier.urihttps://doi.org/10.1016/j.aca.2018.07.055
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10611
dc.identifier.volume1039
dc.identifier.wosWOS:000447155200004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject6-Phosphonohexanoic acid
dc.subjectInterlekin-1 beta
dc.subjectIndium tin oxide
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSingle frequency impedance
dc.subjectSelf-Assembled Monolayer
dc.subjectC-Reactive Protein
dc.subjectGold Nanoparticles
dc.subjectImmunosensor
dc.subjectInterleukin-1-Beta
dc.subjectBiomarker
dc.subjectPolymer
dc.subjectFilm
dc.subjectImmobilization
dc.subjectFabrication
dc.titleA disposable and ultrasensitive ITO based biosensor modified by 6-phosphonohexanoic acid for electrochemical sensing of IL-1 beta in human serum and saliva
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
10611.pdf
Boyut:
180.04 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu