Ultrasensitive determination of cadherin-like protein 22 with a label-free electrochemical immunosensor using brush type poly(thiophene-g-glycidylmethacrylate) modified disposable ITO electrode

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dc.authorid0000-0002-8716-9970
dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, MUHAMMET/ABA-9027-2020
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:17Z
dc.date.available2022-05-11T14:48:17Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA label-free immunosensor for the determination of Cadherin-like protein 22 (CDH 22) was successfully developed by using poly(thiophene-g-glycidyl methacrylate) (PT-GMA) brush type polymer modified disposable ITO electrode. This immunosensor was fabricated by immobilizing of anti-CDH 22 antibodies on epoxy functional groups of brush type polymer. Brush type polymer was coated on the disposable ITO electrode by utilizing the spin-coating method. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) analyses were employed for monitoring of electrochemical properties of the immunosensor. Single frequency impedance (SFI) analysis was utilized to investigate the specific recognition interaction between anti-CDH22 antibody and CDH22 antigen. Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy were used to prove the attachment of anti-CDH 22 antibodies on the electrode surface. Atomic force microscopy (AFM) and Scanning electron microscopy (SEM) analyses were carried out to indicate the changes formed on the sensing interface during the immobilization process. The electrochemical signals in response to different concentrations of CDH 22 antigens were evaluated with EIS measurements under the optimal experimental conditions. With the increase of concentrations of CDH 22 antigens, a thick protein layer was obtained on the electrode surface, resulting in higher electrochemical signals. The variations in impedance values were proportional to the CDH22 concentrations, with a wide linear detection range of 0.01-3 pg/mL, and a low detection limit of 3.2 fg/mL. The suggested immunosensor was an easy and low-cost label-free detection technique and provided a simple way for determination of cancer biomarkers such as CDH 22.
dc.description.sponsorshipTekirdag Namik Kemal University, NKU-BAP [NKUBAP.00.DS.18.180]
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council by Tekirdag Namik Kemal University, NKU-BAP (Grant number: NKUBAP.00.DS.18.180).
dc.identifier.doi10.1016/j.talanta.2019.03.082
dc.identifier.endpage397
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid31036200
dc.identifier.scopus2-s2.0-85063372997
dc.identifier.scopusqualityQ1
dc.identifier.startpage387
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2019.03.082
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10622
dc.identifier.volume200
dc.identifier.wosWOS:000468715300048
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBrush type polymer
dc.subjectElectrochemical impedance spectroscopy
dc.subjectCyclic voltammetry
dc.subjectCadherin-like protein 22
dc.subjectReduced Graphene Oxide
dc.subjectImpedance Spectroscopy
dc.subjectRaman-Spectroscopy
dc.subjectBiosensor
dc.subjectCopolymers
dc.subjectAlpha
dc.subjectAtrp
dc.subjectChallenges
dc.subjectPolymer
dc.subjectSerum
dc.titleUltrasensitive determination of cadherin-like protein 22 with a label-free electrochemical immunosensor using brush type poly(thiophene-g-glycidylmethacrylate) modified disposable ITO electrode
dc.typeArticle

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