The development of an ultra-sensitive electrochemical immunosensor using a PPyr-NHS functionalized disposable ITO sheet for the detection of interleukin 6 in real human serums

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:19Z
dc.date.available2022-05-11T14:48:19Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA label-free impedimetric immunosensor based on a conjugated poly(pyrroleN-hydroxy succinimide) (PPyr-NHS) polymer covered disposable indium tin oxide electrode (ITO) was fabricated for the ultrasensitive determination of interleukin 6 (IL 6) antigen. In this sensing platform, the PPyr-NHS polymer, which carried a number of succinimide groups on its end side, was used as a matrix material for the first time. This synthesized polymer had excellent biocompatibility and good electrical conductivity. In addition, the utilization of this material as a matrix material provided direct immobilization of the IL 6 receptor (IL6R), which was employed as a biorecognition element. The preparation of the working electrode, the successful attachment of IL 6R and specific interaction between IL6R and IL 6 antigen were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Moreover, the working electrode surface was characterized by Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy during its construction. An impedimetric method was employed for the quantification of IL 6 antigen. Under optimized experimental conditions, the designed sensing platform could detect IL 6 antigen with a wide detection range from 0.03 pg mL(-1)to 22.5 pg mL(-1)with a relatively low detection limit of 10.2 fg mL(-1). In addition, the developed biosensor had outstanding sensitivity and specificity, good repeatability and reproducibility, high stability and reusability. Additionally, the designed sensing tool was successfully used in human serum samples. Consequently, the suggested immunosensor was clinically useful in the early detection of prostate cancer by direct determination of the serum IL 6 antigen level after simple dilution.
dc.description.sponsorshipScientific and Technological Research Council of by Tekirdag; Namik Kemal University, NKU-BAPNamik Kemal University [NKUBAP.00.ONAP.19.209]
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirdag. Namik Kemal University, NKU-BAP (Grant number: NKUBAP.00.ONAP.19.209).
dc.identifier.doi10.1039/d0nj03183f
dc.identifier.endpage14238
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue33en_US
dc.identifier.scopus2-s2.0-85090032329
dc.identifier.scopusqualityQ2
dc.identifier.startpage14228
dc.identifier.urihttps://doi.org/10.1039/d0nj03183f
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10625
dc.identifier.volume44
dc.identifier.wosWOS:000562010300035
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofNew Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLabel-Free
dc.subjectSelective Detection
dc.subjectCancer
dc.subjectImmobilization
dc.subjectAptasensor
dc.subjectBiomarkers
dc.subjectBiosensors
dc.subjectGold
dc.subjectQuantification
dc.subjectNanoparticles
dc.titleThe development of an ultra-sensitive electrochemical immunosensor using a PPyr-NHS functionalized disposable ITO sheet for the detection of interleukin 6 in real human serums
dc.typeArticle

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