Lipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein engineering

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dc.contributor.authorVardar-Yel, Nurcan
dc.contributor.authorTutuncu, Havva Esra
dc.contributor.authorSurmeli, Yusuf
dc.date.accessioned2024-10-29T17:58:26Z
dc.date.available2024-10-29T17:58:26Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractLipases are remarkable biocatalysts, adept at catalyzing the breakdown of diverse compounds into glycerol, fatty acids, and mono- and di -glycerides via hydrolysis. Beyond this, they facilitate esterification, transesterification, alcoholysis, acidolysis, and more, making them versatile in industrial applications. In industrial processes, lipases that exhibit high stability are favored as they can withstand harsh conditions. However, most native lipases are unable to endure adverse conditions, making them unsuitable for industrial use. Protein engineering proves to be a potent technology in the development of lipases that can function effectively under challenging conditions and fulfill criteria for various industrial processes. This review concentrated on new trends in protein engineering to enhance the diversity of lipase genes and employed in silico methods for predicting and comprehensively analyzing target mutations in lipases. Additionally, key molecular factors associated with industrial characteristics of lipases, including thermostability, solvent tolerance, catalytic activity, and substrate preference have been elucidated. The present review delved into how industrial traits can be enhanced through directed evolution (epPCR, gene shuffling), rational design (FRESCO, ASR), combined engineering strategies (i.e. CAST, ISM, and FRISM) as protein engineering methodologies in contexts of biodiesel production, food processing, and applications of detergent, pharmaceutics, and plastic degradation.
dc.description.sponsorshipTekirdag Namimath;k Kemal University; Malatya Turgut Ozal University; Altimath;nbas University
dc.description.sponsorshipThe author would like to thank Tekirdag Nam & imath;k Kemal University, Malatya Turgut Ozal University and Alt & imath;nbas University for their contribution to the access of the full-text articles.
dc.identifier.doi10.1016/j.ijbiomac.2024.132853
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid38838897
dc.identifier.scopus2-s2.0-85195461683
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.132853
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14315
dc.identifier.volume273
dc.identifier.wosWOS:001261090700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIndustrial application
dc.subjectEngineering lipase
dc.subjectRecent trends in protein engineering
dc.titleLipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein engineering
dc.typeReview Article

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