A Label-free Electrochemical Immunosensor for Highly Sensitive Detection of TNF alpha, Based on Star Polymer-modified disposable ITO Electrode

Basit Öğe Kaydı
dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:19Z
dc.date.available2022-05-11T14:48:19Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractBackground: Biomarkers are very important disease-related biomolecules which should be analyzed sensitive and selective in related physiological fluids or tissues. Tumor necrosis factor-alpha is a type of cytokine which plays vitlly important roles in different methabolic pathways such as cell death, survival, differentiation, proliferation and migration, and infectious and inflammatory diseases including rheumatoid arthritis, diabetes. Objective: In this study, it was aimed to develop a reliable tool based on star-shaped poly(glycidyl methacrylate) polymer coated disposable indium tin oxide electrode for determination of Tumor necrosis factor-alpha, an important disease biomarker. Methods: Star shaped polymer was used as an interface material for anti- Tumor necrosis factor alpha antibodies immobilization. The antibodies were immobilized covalently onto polymer coated indium tin oxide electrode. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were used for all electrochemical measurements. Results: The suggested immunosensor exhibited a linear range between 0.02 and 4 pg/mL Tumor necrosis factor-alpha, and the detection limit was found as 6 fg/mL. Scanning electron microscopy and atomic force microscopy were used for electrode surface characterization. In addition, the suggested immunosensor was used for Tumor necrosis factor-alpha sensing in human serum samples. The results displayed recoveries between 97.07 and 100.19%. Moreover, this immunosensor had a simple fabrication procedure and a long storage-stability. Conclusion: A new biosensor based on a Star shaped polymer for the ultra sensitive determination of a biomarker Tumor necrosis factor-alpha was developed. The biosensor presented excellent repeatability and reproducubility, and also wide calibration range for Tumor necrosis factor-alpha.
dc.identifier.doi10.2174/1573412916999200409111759
dc.identifier.endpage459
dc.identifier.issn1573-4129
dc.identifier.issn1875-676X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85099702364
dc.identifier.scopusqualityQ3
dc.identifier.startpage450
dc.identifier.urihttps://doi.org/10.2174/1573412916999200409111759
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10626
dc.identifier.volume17
dc.identifier.wosWOS:000614254400010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCurrent Pharmaceutical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTumor necrosis factor alpha
dc.subjectelectrochemical immunosensor
dc.subjectsingle frequencies impedance
dc.subjectstar poly(glycidyl methacrylate) polymer
dc.subjectNecrosis-Factor-Alpha
dc.subjectBiosensor Platform
dc.subjectHuman Saliva
dc.subjectHuman Serum
dc.subjectInterleukin-1-Beta
dc.titleA Label-free Electrochemical Immunosensor for Highly Sensitive Detection of TNF alpha, Based on Star Polymer-modified disposable ITO Electrode
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu