A sensitive and selective approach for detection of IL 1? cancer biomarker using disposable ITO electrode modified with epoxy-substituted polythiophene polymer

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dc.authorscopusid55571487900
dc.contributor.authorAydın, Muhammet
dc.date.accessioned2022-05-11T14:48:16Z
dc.date.available2022-05-11T14:48:16Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA highly sensitive and label-free electrochemical immunosensor for sensitive detection of interleukin 1? cancer biomarker was described by using epoxy-substituted polythiophene polymer modified disposable indium tin oxide electrode. This conjugated polymer was synthesized to fabricate this immunosensor for the first time and it offered a good biosensing platform for anti-IL 1? antibody immobilization due to epoxy groups present on the side groups of the polymer. Furthermore, the epoxy-substituted polythiophene polymer coated indium tin oxide electrode was used for the determination of IL 1? antigen for the first time. Electrode fabrication stages were characterized by using electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) techniques. Under optimum experimental conditions, impedimetric responses of the immunosensor increased with the increasing of IL 1? antigen concentration, and the proposed immunosensor displayed a wide linear detection range from 0.01 pg/mL to 5.5 pg/mL with a detection limit of 3.4 fg/mL. The proposed immunosensor exhibited outstanding performance including excellent reproducibility, good repeatability, high selectivity and long storage stability. With the advantages of simple operation, low-cost fabrication and label-free format, the suggested immunosensor was expected to have potential applications for IL 1? cancer biomarker detection. © 2019 Elsevier B.V.
dc.identifier.doi10.1016/j.bios.2019.111675
dc.identifier.issn0956-5663
dc.identifier.pmid31518789
dc.identifier.scopus2-s2.0-85071935392
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.bios.2019.111675
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10619
dc.identifier.volume144
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofBiosensors and Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectConjugated polymer
dc.subjectElectrochemical impedance spectroscopy
dc.subjectEpoxy-substituted polythiophene
dc.subjectInterleukin 1?
dc.subjectAntigens
dc.subjectAtomic force microscopy
dc.subjectBiomarkers
dc.subjectConjugated polymers
dc.subjectCyclic voltammetry
dc.subjectDiseases
dc.subjectElectrochemical impedance spectroscopy
dc.subjectImmunosensors
dc.subjectIndium compounds
dc.subjectScanning electron microscopy
dc.subjectSpectroscopy
dc.subjectTin oxides
dc.subjectAntibody immobilization
dc.subjectCancer biomarker detections
dc.subjectElectrochemical immunosensors
dc.subjectElectrode fabrication
dc.subjectExperimental conditions
dc.subjectIndium tin oxide electrodes
dc.subjectInterleukin-1
dc.subjectLinear detection ranges
dc.subjectElectrochemical electrodes
dc.subjectinterleukin 1alpha
dc.subjectpolyethylene terephthalate
dc.subjectpolymer
dc.subjectpolythiophene
dc.subjectunclassified drug
dc.subjectepoxy resin
dc.subjectIL1A protein, human
dc.subjectimmobilized antibody
dc.subjectindium tin oxide
dc.subjectinterleukin 1alpha
dc.subjectpolymer
dc.subjectpolythiophene
dc.subjectthiophene derivative
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectcyclic potentiometry
dc.subjecthuman
dc.subjectimpedance spectroscopy
dc.subjectindium tin oxide electrode
dc.subjectlimit of detection
dc.subjectmeasurement repeatability
dc.subjectreproducibility
dc.subjectscanning electron microscopy
dc.subjectserum
dc.subjectchemistry
dc.subjectgenetic procedures
dc.subjectisolation and purification
dc.subjectneoplasm
dc.subjectprocedures
dc.subjectAntibodies, Immobilized
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectDielectric Spectroscopy
dc.subjectEpoxy Resins
dc.subjectHumans
dc.subjectInterleukin-1alpha
dc.subjectNeoplasms
dc.subjectPolymers
dc.subjectThiophenes
dc.subjectTin Compounds
dc.titleA sensitive and selective approach for detection of IL 1? cancer biomarker using disposable ITO electrode modified with epoxy-substituted polythiophene polymer
dc.typeArticle

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